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| + | =Solubility= |
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| + | Ifenprodil is not very solubile in aqueos solutions. However, because it has an amine group, it can be made a solubile salt when mixed with an organic acid such as tartaric acid. |
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− | Solubility;
| + | Here is our recipe (February 2008): |
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| + | ''(Note that we have a final solution with 1% tartaric acid, and most people seem to end up with 0.1%. Probably I was just impatient and so used a high concentration of tartaric acid to guarantee dissolution.)'' |
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| Prepare 10% tartaric acid made in distilled water, | | Prepare 10% tartaric acid made in distilled water, |
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− | e.g. 1 g tartaric acid in 10 ml final volume. | + | :e.g. 1 g tartaric acid in 10 ml final volume. |
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| Dissolve ifenprodil in 10% tartaric acid at 10x the desired final concentration | | Dissolve ifenprodil in 10% tartaric acid at 10x the desired final concentration |
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− | e.g. if you want a final concentration of 3 mg/ml, then dissovle 30 mg ifenprodil in 1 ml of 10% tartaric acid. | + | :e.g. if you want a final concentration of 3 mg/ml, then dissovle 30 mg ifenprodil in 1 ml of 10% tartaric acid. |
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| Then add 0.1M Phosphate buffer, 0.15M NaCl to bring the solution to the desired final concentration | | Then add 0.1M Phosphate buffer, 0.15M NaCl to bring the solution to the desired final concentration |
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− | e.g. add 9 ml of PBS to 30 mg ifenprodil dissolved in 1 ml of 10% tartaric acid | + | :e.g. add 9 ml of PBS to 30 mg ifenprodil dissolved in 1 ml of 10% tartaric acid |
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| Bring the pH of the solution from ~pH 4 to ~ pH 7 by added a small amount of 10 N NaOH | | Bring the pH of the solution from ~pH 4 to ~ pH 7 by added a small amount of 10 N NaOH |
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− | e.g., add 10 N NaOH only 10 microliters at a time, checking pH with pH paper until the solution is neutralized. | + | :e.g., add 10 N NaOH only 10 microliters at a time, checking pH with pH paper until the solution is neutralized. |
− | it takes about 100 microliters of 10N NaOH to neutralize 10 ml of 1% tartaric acid, but add the NaOH bit by bit and check pH frequently.
| + | :''it takes about 100 microliters of 10N NaOH to neutralize 10 ml of 1% tartaric acid, but add the NaOH bit by bit and check pH frequently.'' |
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| Prepare vehicle of 0.1M PBS, 1% tartaric acid and pH to 7.0 as above. | | Prepare vehicle of 0.1M PBS, 1% tartaric acid and pH to 7.0 as above. |
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− | e.g. mix 1 ml of 10% tartaric acid and 9 ml of 0.1M PBS, use 10 N NaOH to bring to pH 7.0. | + | :e.g. mix 1 ml of 10% tartaric acid and 9 ml of 0.1M PBS, use 10 N NaOH to bring to pH 7.0. |
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