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'''Lateral Ventricular Cannula:'''
1.2 mm caudal to bregma
1.5 mm lateral to the midline
4 mm below the skull surface
'''Method'''
Each rat was stereotaxically implanted with a 22 gauge, stainless steel, guide
cannula (Plastics One, Roanoke, VA) aimed towards the
lateral cerebral ventricle (1.2 mm caudal to bregma, 1.5 mm
lateral to the midline, and 4 mm below the skull surface).
Guide cannulas were held in place with dental acrylic
bonded to stainless steel screws anchored to the skull. An
obdurator was inserted into each guide cannula and remained
in place except during injections when it was removed
and replaced with an injector that extended 1.0 mm
beyond the tip of the guide cannula.