1,142
edits
Changes
created and added DAAO
RT-PCR Conditions for a bunch of genes implicated in Bipolar Disorder
=DAAO=
Source: Konno 1998 PMID 12734589
Sense primer (F: 5’-GGTTAACTGAGAGGGGAGTGAA-3’)
Antisense primer (R: 5’- CCATAGTTGTGGATGACCTCTG-3’)
designed from the sequence conserved in cDNAs encoding human, mouse, rabbit, and pig D-amino-acid oxidases.
initial denaturation at 94°C for 1 min,
30 cycles of PCR
:denaturation: 94°C, 30 sec
:annealing: 55°C, 30 sec
:extension: 72°C, 45 sec)
final extension was done at 72°C for 5 min
=DAAO=
Source: Konno 1998 PMID 12734589
Sense primer (F: 5’-GGTTAACTGAGAGGGGAGTGAA-3’)
Antisense primer (R: 5’- CCATAGTTGTGGATGACCTCTG-3’)
designed from the sequence conserved in cDNAs encoding human, mouse, rabbit, and pig D-amino-acid oxidases.
initial denaturation at 94°C for 1 min,
30 cycles of PCR
:denaturation: 94°C, 30 sec
:annealing: 55°C, 30 sec
:extension: 72°C, 45 sec)
final extension was done at 72°C for 5 min