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→Mini-Pumps
:Osmotic minipumps (Model 2001, Alzet, Alza, Palo Alto, CA) were implanted in the peritoneal cavity of animals under halothane anesthesia. For half of the animals in each diet group ... the minipumps contained 200 ul of a saturated aqueous solution of LiCl (63 g/100 ml). The pumps delivered a relatively constant ip infusion of solution 1 ul per hour) for approximately 9 days.
:Osmotic minipumps (Model 2001, Alzet, Alza, Palo Alto, CA) were implanted in the peritoneal cavity of animals under halothane anesthesia. For half of the animals in each diet group ... the minipumps contained 200 ul of a saturated aqueous solution of LiCl (63 g/100 ml). The pumps delivered a relatively constant ip infusion of solution 1 ul per hour) for approximately 9 days.
For Lee Carella's experiment:
Dose: 3 mmol/kg/day.
Body weight: 0.3 kg
LiCl /rat/ day: 0.9 mmol /rat/ day
Flow rate: 0.25 ul / hour (Alzet minipump model 2004)
Flow rate: 6 ul / day
LiCl concentration in pump: 0.9 mmol / 6 ul = 0.9 mol / 6 ml = 150 M !!!
Maximum Concentration of LiCl in the pump: 15 M = 63 g / 100 ml
Flow Rate = 6 ul / day
LiCl Dose / day = 90 umol