Changes

Ibotenic acid (10 mg/ml in saline) will be infused over 5 min for a total volume of 0.1 µl.

Ibotenic acid (10 mg/ml in saline) will be infused over 5 min for a total volume of 0.1 µl.

Rats will be stereotaxically injected with ibotenic acid (Natural Products Co., Vashon, WA) to destroy cell bodies within specific brain subnuclei while sparing fibers of passage.  Under isoflurane anesthesia and following aseptic surgical preparation, the rat's head is immobilized in a stereotaxic apparatus. An incision is made longitudinally along the midline of the head, to expose the skull.  A 1.8 mm hole is drilled in the skull with a Dremel motortool and a Fine Science Tool trephine bit.  Injections will be made through a blunt 33 gauge needle attached to a 2 µl Hamilton microsyringe under the control of a syringe pump.  Ibotenic acid (10 mg/ml in saline) will be infused over 5 min for a total volume of 0.1 µl.  The injector needle will be left in place for 10 min after the infusion and before withdrawal to minimize back flow along the needle track.  Sham lesions will be produced by injection of phosphate-buffered saline (0.1 µl) into the same areas, thus controlling for non-specific damage from lowering the injector through overlying tissue.  All lesions will be produced bilaterally.   

Rats will be stereotaxically injected with ibotenic acid (Natural Products Co., Vashon, WA) to destroy cell bodies within specific brain subnuclei while sparing fibers of passage.  Under isoflurane anesthesia and following aseptic surgical preparation, the rat's head is immobilized in a stereotaxic apparatus. An incision is made longitudinally along the midline of the head, to expose the skull.  A 1.8 mm hole is drilled in the skull with a Dremel motortool and a Fine Science Tool trephine bit.  Injections will be made through a blunt 33 gauge needle attached to a 2 µl Hamilton microsyringe under the control of a syringe pump.  Ibotenic acid (10 mg/ml in saline) will be infused over 5 min for a total volume of 0.1 µl.  The injector needle will be left in place for 10 min after the infusion and before withdrawal to minimize back flow along the needle track.  Sham lesions will be produced by injection of phosphate-buffered saline (0.1 µl) into the same areas, thus controlling for non-specific damage from lowering the injector through overlying tissue.  All lesions will be produced bilaterally.