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added note on use of anti NeuN
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Prior to experimental testing, coordinates for injector placement will be determined in a group of test animals based on Paxinos and WatsonInjector placement will be verified by injections of Sudan Black (0.4 µl). A series of preliminary studies will be conducted to determine the extent of cell damage by cresyl violet staining. Animals will have injections performed, then be euthanized. Sections will be cut through the remainder of the brain and stained with Weil myelin stain to visualize degenerating neurons that may project through the lesion site. Based on these preliminary studies, the concentration and volume of ibotenic acid injections will be adjusted to maximize cell death without damaging fibers of passage.
Prior to experimental testing, coordinates for injector placement will be determined in a group of test animals based on Paxinos and WatsonInjector placement will be verified by injections of Sudan Black (0.4 µl). A series of preliminary studies will be conducted to determine the extent of cell damage by cresyl violet staining. Animals will have injections performed, then be euthanized. Sections will be cut through the remainder of the brain and stained with Weil myelin stain to visualize degenerating neurons that may project through the lesion site. Based on these preliminary studies, the concentration and volume of ibotenic acid injections will be adjusted to maximize cell death without damaging fibers of passage.
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+=Verification of Lesion Site=
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+Following Jongen-Relo, A. L., & Feldon, J. (2002) PMID 12126979 and in the gustatory cortex, Geddes et al. (2008) PMID 18823161, we will determine loss of neurons using a mouse monoclonal [http://www.millipore.com/catalogue/item/mab377 anti-NeuN, clone A60], from Millipore, catalog MAB377, price $259.00.
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[[Category:Taste Aversion]]
[[Category:Taste Aversion]]