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added NeuN antisera concentration
Line 21:
Line 21:
=Verification of Lesion Site=
=Verification of Lesion Site=
−Following Jongen-Relo, A. L., & Feldon, J. (2002) PMID 12126979 and in the gustatory cortex, Geddes et al. (2008) PMID 18823161, we will determine loss of neurons using a mouse monoclonal [http://www.millipore.com/catalogue/item/mab377 anti-NeuN, clone A60], from Millipore, catalog MAB377, price $259.00.
+Following Jongen-Relo, A. L., & Feldon, J. (2002) PMID 12126979 and in the gustatory cortex, Geddes et al. (2008) PMID 18823161, we will determine loss of neurons using a mouse monoclonal [http://www.millipore.com/catalogue/item/mab377 anti-NeuN, clone A60], from Millipore, catalog MAB377, price $259.00. Jongen-Relo used a primary concentration of 1:5000 with a 48-h incubation at 4 C.
[[Category:Taste Aversion]]
[[Category:Taste Aversion]]