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added BRDU labeling refs
Line 42:
Line 42:
#Staining by DAB kit (Vector).
#Staining by DAB kit (Vector).
#Rinse 5 X 5 min in 0.1M PBS.
#Rinse 5 X 5 min in 0.1M PBS.
+
+
+==DNA Labeling with BrdUTP==
+
+Use bromodeoxyuridine triphosphate as one of the dNTPs in labeling reaction, then stain with anti-BrdU antibodies
=Radiation Sensitization=
=Radiation Sensitization=
Incorporation of BrdU makes cells more sensitive to radiation damage.
Incorporation of BrdU makes cells more sensitive to radiation damage.
+
+== Toxicity==
+
+Meuth & Green PMID 4477047
+"Bromodeoxyuridine triphosphate is an allosteric inhibitor of ribonucleotide reductase, and bromodeoxyuridine can therefore kill cells by starving them for deoxycytidine nucleotides."
+
+PMID 18770731, PMID 8555370 PMID 8871927 PMID 1726743
=Pharmacokinetics=
=Pharmacokinetics=