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added BRDU labeling refs
#Staining by DAB kit (Vector).
#Staining by DAB kit (Vector).
#Rinse 5 X 5 min in 0.1M PBS.
#Rinse 5 X 5 min in 0.1M PBS.
==DNA Labeling with BrdUTP==
Use bromodeoxyuridine triphosphate as one of the dNTPs in labeling reaction, then stain with anti-BrdU antibodies
=Radiation Sensitization=
=Radiation Sensitization=
Incorporation of BrdU makes cells more sensitive to radiation damage.
Incorporation of BrdU makes cells more sensitive to radiation damage.
== Toxicity==
Meuth & Green PMID 4477047
"Bromodeoxyuridine triphosphate is an allosteric inhibitor of ribonucleotide reductase, and bromodeoxyuridine can therefore kill cells by starving them for deoxycytidine nucleotides."
PMID 18770731, PMID 8555370 PMID 8871927 PMID 1726743
=Pharmacokinetics=
=Pharmacokinetics=