Changes

*to linearize cDNAs of interest for the preparation of cDNA blot

*to linearize cDNAs of interest for the preparation of cDNA blot

1. you may want to have three different concentrations of each cDNA on your blot,  

1. you may want to have three different concentrations of each cDNA on your blot, like 20ng, 100ng, and 500ng.

  like 20ng, 100ng, and 500ng.

2. so you would need 1ug of  each plasmid for 1 set of cDNA blot, and your blotter is big  

2. so you would need 1ug of  each plasmid for 1 set of cDNA blot, and your blotter is big enough to make 2 sets of cDNA blots at a time, so set up the digestion with 2ug of each plasmid.

  enough to make 2 sets of cDNA blots at a time, so set up the digestion with 2ug of each

  plasmid.

3. The basic formular for the digestion mixture is

3. The basic formular for the digestion mixture is