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==Dr. Yi Zhou's Thy1-R18 Transgenic Mice==
See SFN abstracts (2010) 53.1/M17 and (2011) 790.09/FF16
From Courtney Graham's Honor's Thesis:
In order to study the role of 14-3-3 proteins in the nervous system_ in
vivo_, we have successfully created lines of neuronal-specific
transgenic mice that may inhibit 14-3-3 dependent cellular processes.
These transgenic mice express a YFP-fused peptide (R18) which binds to
endogenous 14-3-3, and thus blocking its capability to interact with its
binding partners; and are therefore considered to be a functional
knockout. The transgenes are expressed under the control of the Thy-1
promoter which is neuronal-specific and produces variation of transgene
expression in different brain regions, which is great among founder
lines but minimal among descendants of a single founder (Feng et al.
2000). To minimize strain-dependent genetic variation, we have
backcrossed the founder lines with wild-type C57BL/6 mice for at least 8
generations.
R18 peptide paper: PMID 10493820
Thy-1 Promoter paper: PMID 11086982
info on Dr. Zhou's mice
==Dr. Yi Zhou's Thy1-R18 Transgenic Mice==
See SFN abstracts (2010) 53.1/M17 and (2011) 790.09/FF16
From Courtney Graham's Honor's Thesis:
In order to study the role of 14-3-3 proteins in the nervous system_ in
vivo_, we have successfully created lines of neuronal-specific
transgenic mice that may inhibit 14-3-3 dependent cellular processes.
These transgenic mice express a YFP-fused peptide (R18) which binds to
endogenous 14-3-3, and thus blocking its capability to interact with its
binding partners; and are therefore considered to be a functional
knockout. The transgenes are expressed under the control of the Thy-1
promoter which is neuronal-specific and produces variation of transgene
expression in different brain regions, which is great among founder
lines but minimal among descendants of a single founder (Feng et al.
2000). To minimize strain-dependent genetic variation, we have
backcrossed the founder lines with wild-type C57BL/6 mice for at least 8
generations.
R18 peptide paper: PMID 10493820
Thy-1 Promoter paper: PMID 11086982