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In Situ Hybridization (view source)
Revision as of 14:50, 22 November 2011
, 14:50, 22 November 2011→Reagents and Solutions: added formamide
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20x SSC Buffer 20x Ultrapure, 1L bottle, Invitrogen #15557-044 $34+
−Denhardt's Solution 50x, 100 ml, Invitrogen # 750018 $122
−:(made from PVP (polyvinyl pyrrolidone) 40 & Ficoll Type 400)+DTT (dithiothreitol) (aka Cleland's reagent) Ultrapure, 5 g, Invitrogen # 15508013 $102.00+
''prices as of 2011-11''
''prices as of 2011-11''
−DEPC (diethylpyrocarbonate), Sigma #D-5758-50 ml $203
+Denhardt's Solution 50x, 100 ml, Invitrogen # 750018 $122
+:(made from PVP (polyvinyl pyrrolidone) 40 & Ficoll Type 400)DEPC (diethylpyrocarbonate), Sigma #D-5758-50 ml $203
Dextran Sulfate sodium salt, Sigma #D-8906-50 g, $143
Dextran Sulfate sodium salt, Sigma #D-8906-50 g, $143
−DTT (dithiothreitol) (aka Cleland's reagent) Ultrapure, 5 g, Invitrogen #15508013 $102.00
−Formamide Ultrapure, 500g bottle, Invitrogen #15515026 $97
Salmon Sperm DNA 10 mg/ml, 10 x 1 ml tubes, Invitrogen #AM9680 $198
Salmon Sperm DNA 10 mg/ml, 10 x 1 ml tubes, Invitrogen #AM9680 $198
−SSC Buffer 20x Ultrapure, 1L bottle, Invitrogen #15557-044 $34
−==Methods Section==
==Methods Section==