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JB Systemic Sodium Selenate (view source)
Revision as of 13:35, 29 November 2011
, 13:35, 29 November 2011Created agenda
(Brief background of the experiment).
[[Selenium|Sodium Selenate]] (Na2Se04) activates PP2A.
==Agenda==
'''1. Test Conditioned Taste Aversion (23 days)'''
Rats (n= 24) on 8 days of water restriction (3h, 3h, 1h, 1h, 30 min, 30 min, 10 min, 10 min)
Conditioning day:
4 groups (n=6 per group) : NaCl/NaCl, NaCl/LiCl, Na2Se04/NaCl, Na2Se04/LiCl
at -2 h, Inject with sodium selenate 12 mg/kg i.p. (n=12) or NaCl 0.15 M , 1 ml/kg (n= 12)
at 0 h, 10 min access to 0.125% saccharin (CS)
at 0.5 h, inject with LiCl (0.15 M, 6 ml/kg, i.p.) (n=12) or NaCl (n=12)
at 1.5 h, Return water overnight
Next day start 2-bottle, 24-h preference test (saccharin vs. water) for 14 days.
'''2. Test c-Fos induction'''
reuse same rats, shuffled into 4 groups: NaCl/NaCl, NaCl/LiCl, Na2Se04/NaCl, Na2Se04/LiCl
at -2 h, Inject with sodium selenate 12 mg/kg i.p. (n=12) or NaCl 0.15 M , 1 ml/kg (n= 12)
at 0 h, inject with LiCl (0.15 M, 6 ml/kg, i.p.) (n=12) or NaCl (n=12)
at 1 h, perfuse rats
process for c-Fos (~ 1 week in the lab)
[[Selenium|Sodium Selenate]] (Na2Se04) activates PP2A.
==Agenda==
'''1. Test Conditioned Taste Aversion (23 days)'''
Rats (n= 24) on 8 days of water restriction (3h, 3h, 1h, 1h, 30 min, 30 min, 10 min, 10 min)
Conditioning day:
4 groups (n=6 per group) : NaCl/NaCl, NaCl/LiCl, Na2Se04/NaCl, Na2Se04/LiCl
at -2 h, Inject with sodium selenate 12 mg/kg i.p. (n=12) or NaCl 0.15 M , 1 ml/kg (n= 12)
at 0 h, 10 min access to 0.125% saccharin (CS)
at 0.5 h, inject with LiCl (0.15 M, 6 ml/kg, i.p.) (n=12) or NaCl (n=12)
at 1.5 h, Return water overnight
Next day start 2-bottle, 24-h preference test (saccharin vs. water) for 14 days.
'''2. Test c-Fos induction'''
reuse same rats, shuffled into 4 groups: NaCl/NaCl, NaCl/LiCl, Na2Se04/NaCl, Na2Se04/LiCl
at -2 h, Inject with sodium selenate 12 mg/kg i.p. (n=12) or NaCl 0.15 M , 1 ml/kg (n= 12)
at 0 h, inject with LiCl (0.15 M, 6 ml/kg, i.p.) (n=12) or NaCl (n=12)
at 1 h, perfuse rats
process for c-Fos (~ 1 week in the lab)