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mLine 35:
Line 35: − − Plastics One Cannula: Line 43:
Line 41: − + − + Line 53:
Line 51: − + − + −
→Fourth Ventricular Cannula:
7.2 mm below the skull surface
7.2 mm below the skull surface
===Method===
===Method===
was positioned on the midline 3.2 mm caudal to lambda and 7.2 mm below the skull surface (stereotaxic coordinates
was positioned on the midline 3.2 mm caudal to lambda and 7.2 mm below the skull surface (stereotaxic coordinates
taken from (Baird et al. 2009 PMID 19008313)). Guide cannulas were held in place with dental acrylic bonded to stainless
taken from (Baird et al. 2009 PMID 19008313)). Guide cannulas were held in place with dental acrylic bonded to stainless
steel screws anchored to the skull. An obdurator (model C315DC; Plastics One, Inc.) extending 1.0 mm beyond the guide
steel screws anchored to the skull. A 26-gauge obdurator extending 1.0 mm beyond the guide
cannula was inserted into each guide cannula and remained in place except during injections when it was removed and
cannula was inserted into each guide cannula and remained in place except during injections when it was removed and
replaced with a XXX-gauge injector that extended 1.0 mm beyond the tip of the guide cannula.
replaced with a 26-gauge injector that extended 1.0 mm beyond the tip of the guide cannula.
===Test===
===Test===
Immediately after the ICV injection, rats were returned to their home cage with access to a water bottle.
Immediately after the ICV injection, rats were returned to their home cage with access to a water bottle.
The latency to initiate and duration of grooming was recorded; rats that failed to groom within 2 minutes of the
The latency to initiate and duration of grooming was recorded; rats that failed to groom within 2 minutes of the
bombesin injection or groomed for less than XXX minutes were dropped from the study. Cannula placements were
bombesin injection or groomed for less than XXX minutes were dropped from the study. Cannula placements were
also verified postmortem by sectioning through the brain.
also verified postmortem by sectioning through the brain.
==Supplies==
==Supplies==