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ICV Cannulation
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Revision as of 13:06, 12 September 2012
No change in size
,
13:06, 12 September 2012
m
→Method
Line 41:
Line 41:
was positioned on the midline 3.2 mm caudal to lambda and 7.2 mm below the skull surface (stereotaxic coordinates
was positioned on the midline 3.2 mm caudal to lambda and 7.2 mm below the skull surface (stereotaxic coordinates
taken from (Baird et al. 2009 PMID 19008313)). Guide cannulas were held in place with dental acrylic bonded to stainless
taken from (Baird et al. 2009 PMID 19008313)). Guide cannulas were held in place with dental acrylic bonded to stainless
−
steel screws anchored to the skull. A
26
-gauge obdurator extending 1.0 mm beyond the guide
+
steel screws anchored to the skull. A
28
-gauge obdurator extending 1.0 mm beyond the guide
cannula was inserted into each guide cannula and remained in place except during injections when it was removed and
cannula was inserted into each guide cannula and remained in place except during injections when it was removed and
−
replaced with a
26
-gauge injector that extended 1.0 mm beyond the tip of the guide cannula.
+
replaced with a
28
-gauge injector that extended 1.0 mm beyond the tip of the guide cannula.
===Test===
===Test===
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