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For other stereotaxic coordinates, see also [[Amygdala]], [[JA Lateral Hypothalamus Lesions]].
For other stereotaxic coordinates, see also [[Amygdala]], [[JA Lateral Hypothalamus Lesions]].
==Lateral Ventricular Cannula:==
==Lateral Ventricular Cannula==
1.2 mm caudal to bregma
1.2 mm caudal to bregma
4 mm below the skull surface
4 mm below the skull surface
Under isoflurane anesthesia, each rat was stereotaxically implanted with a 22-gauge, stainless steel, guide
Under isoflurane anesthesia, each rat was stereotaxically implanted with a 22-gauge, stainless steel, guide
except during injections when it was removed and replaced with an 28-gauge injector that extended 1.0 mm beyond the tip of the guide cannula.
except during injections when it was removed and replaced with an 28-gauge injector that extended 1.0 mm beyond the tip of the guide cannula.
To test the patency and placement of the cannula in the lateral ventricle, water-replete rats were injected with
To test the patency and placement of the cannula in the lateral ventricle, water-replete rats were injected with
100 ng human angiotensin II (Ang II; Sigma Chemical Co, St Louis, MO) dissolved in 5 µl volume of saline (0.15 M).
100 ng human angiotensin II (Ang II; Sigma Chemical Co, St Louis, MO) dissolved in 5 µl volume of saline (0.15 M).
ICV injections of 5 µl volume were given by hand to lightly restrained rats using a 50 µl Hamilton microsyringe.
ICV injections of 5 µl volume were given by hand to lightly restrained rats using a 50 µl Hamilton microsyringe.
==Fourth Ventricular Cannula:==
==Fourth Ventricular Cannula==
3.2 mm caudal to lambda
3.2 mm caudal to lambda
7.2 mm below the skull surface
7.2 mm below the skull surface
Under isoflurane anesthesia, each rat was stereotaxically implanted with a single 22-gauge stainless steel guide cannula
Under isoflurane anesthesia, each rat was stereotaxically implanted with a single 22-gauge stainless steel guide cannula
(model C315G; Plastics One, Inc., Roanoke, VA) aimed towards the fourth ventricle (4V). The tip of the guide cannula
(model C315G; Plastics One, Inc., Roanoke, VA) aimed towards the fourth ventricle (4V). The tip of the guide cannula
replaced with a 28-gauge injector that extended 1.0 mm beyond the tip of the guide cannula.
replaced with a 28-gauge injector that extended 1.0 mm beyond the tip of the guide cannula.
To test the patency and placement of the cannula in the lateral ventricle, water-replete rats were injected with
To test the patency and placement of the cannula in the lateral ventricle, water-replete rats were injected with
XXX ng bombesin (California Peptides) dissolved in 5 µl volume of saline (0.15 M). The volume of all icv injections
XXX ng bombesin (California Peptides) dissolved in 5 µl volume of saline (0.15 M). The volume of all icv injections
''Check prices on annual price list.''
''Check prices on annual price list.''
====22 Gauge guide cannulas for ventricular injections:====
====22 gauge guide cannulas for ventricular injections====
Use C313 components, which provide 28 gauge dummy and injectors.
Use C313 components, which provide 28 gauge dummy and injectors.
:C313I/SPC for 5 mm C313G with 1 mm projection
:C313I/SPC for 5 mm C313G with 1 mm projection
====26 Gauge guide cannulas for site-specific injections====
====26 gauge guide cannulas for site-specific injections====
Use C315 components, which provide 33 gauge dummy and injectors.
Use C315 components, which provide 33 gauge dummy and injectors.
We use screws of 0-80 size with shaft length 3/32 inches (2.4 mm).
We use screws of 0-80 size with shaft length 3/32 inches (2.4 mm).
These are a little long, but they are stronger and easier to work with than 1/16 inch (1.6 mm) long screws.
These are a little long, but they are stronger and easier to work with than 1/16 inch (1.6 mm) long screws.
Also need the small drill pit.
Also need the small drill bit.
:0-80 X 3/32 (packages of 100)
:0-80 X 3/32 (packages of 100)