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Created page with 'Objective 5x Zoom 4.0 x (except PBN as 3.0x) Format 1280x1024 Live (play button icon) Adjust AutoExposure/Analog Gain or enter set exposure time Acquire -> Capture (camera ico…'
Objective 5x
Zoom 4.0 x (except PBN as 3.0x)
Format 1280x1024
Live (play button icon)
Adjust AutoExposure/Analog Gain or enter set exposure time
Acquire -> Capture (camera icon)
captures image with flurophore channel (e.g. "FITC")
(have to restart Live after a capture to view live image again)
Save As -> Tagged Image Format (TIFF)
proprietary Nikon file format that preserves acquisition parameters
Using TIFF files, Drag and drop one channel from one TIFF file onto second channel in second TIFF file to create a merged TIFF file.
e.g., drag FITC on top of TRITC channel
Save merged image as TIFF file
Save As JPEG
Save merged image as JPEG file to convert from emission filter channels to RGB
Zoom 4.0 x (except PBN as 3.0x)
Format 1280x1024
Live (play button icon)
Adjust AutoExposure/Analog Gain or enter set exposure time
Acquire -> Capture (camera icon)
captures image with flurophore channel (e.g. "FITC")
(have to restart Live after a capture to view live image again)
Save As -> Tagged Image Format (TIFF)
proprietary Nikon file format that preserves acquisition parameters
Using TIFF files, Drag and drop one channel from one TIFF file onto second channel in second TIFF file to create a merged TIFF file.
e.g., drag FITC on top of TRITC channel
Save merged image as TIFF file
Save As JPEG
Save merged image as JPEG file to convert from emission filter channels to RGB