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→Sodium Selenate
PubChem Substance ID: [http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi?sid=24899787 24899787]
PubChem Substance ID: [http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi?sid=24899787 24899787]
'''Tonicity of Systemic Injection'''
Osmolarity of Na2Se04 at 12 mg/ml (63 mM) = 189 mOsm
Osmolarity of Na2Se04 at 12 mg/ml (63 mM) = 189 mOsm
So Na2Se04 at 12 mg/ml (63 mM), NaCl at 3.2 mg/ml (55mM) = 300 mOsm
So Na2Se04 at 12 mg/ml (63 mM), NaCl at 3.2 mg/ml (55mM) = 300 mOsm
'''Calculation of suggested i.c.v. injection concentration.'''
Assuming a 500 g rat, then systemic dose is 0.25 mg ( 250 µg). A central dose might be 1/100th of the system dose -> 2.5 µg.
Volume of i.c.v injection would be 5 µl; site specific injection 0.5 µl
So central dose would be 2.5 µg / 5 µl = 0.5 µg/ 1 µl = 0.5 g / L.
MW of sodium selenate is 188.94, so 0.5 g = 2.65 mmole
so central injection dose is 5 µl at 2.65 mM.
(Osmolarity is sufficiently low that can be diluted with saline).
==Toxicity of Selenate==
==Toxicity of Selenate==