Changes

== Procedure ==

== Procedure ==

Male 14-3-3 FKO mice (n=6) and wildtype C57 mice (n=6), singly housed with ad lib access to food and water. Habituated to [[Odor Arena]] (without stimulus dish) for 5 min per day for 4 days. Then exposed to an odor stimuli for 5 min, 1 stimulus/day, for 3 consective days.

Male 14-3-3 FKO mice (n=6) and wildtype C57 mice (n=6), singly housed with ad lib access to food and water. Habituated to [[Odor Arena]] (without stimulus dish) for 5 min per day for 4 days.  

 

 

All mice exposed to an odor stimuli for 5 min, 1 stimulus/day, for 3 consective days.

Odor stimuli are either 5 ul distilled water, 5 ul TMT (1% in water), or 1.25 ul 2PE (neat). Stimuli pipetted onto filter paper (#1 Whatman, 25mm diameter disc) and placed in small glass petri dish. Stimuli were prepared prior to bringing mouse home cage into test room. Stimulus was placed in random corner of the 29cm x 29cm arena immediately prior to mouse being placed in center of arena. Each stimulus was presented once, in random order for each mouse.

Odor stimuli are either 5 ul distilled water, 5 ul TMT (1% in water), or 1.25 ul 2PE (neat). Stimuli pipetted onto filter paper (#1 Whatman, 25mm diameter disc) and placed in small glass petri dish. Stimuli were prepared prior to bringing mouse home cage into test room. Stimulus was placed in random corner of the 29cm x 29cm arena immediately prior to mouse being placed in center of arena. Each stimulus was presented once, in random order for each mouse.

Between mice, the arena was rinsed with soapy water (Alconox) and 70% isopropanol. Stimulus filter paper and pipette tips were disposed into sealed container of bleach.

Between mice, the arena was rinsed with soapy water (Alconox) and 70% isopropanol. Stimulus filter paper and pipette tips were disposed into sealed container of bleach.

==Stimuli==

==Stimuli==