Changes

R18  is a synthetic 21 amino acid unphosphorylated peptide (MW 2309.6)  which binds to the same amphipathic groove on the surface of 14-3-3 as phosphorylated peptides, with high affinity for all 7 mammalian isoforms of 14-3-3 PMID 10493820. Commercial source of peptide [Enzo #BML-P214-0001 http://www.enzolifesciences.com/BML-P214/r18-peptide/].

R18  is a synthetic 21 amino acid unphosphorylated peptide (MW 2309.6)  which binds to the same amphipathic groove on the surface of 14-3-3 as phosphorylated peptides, with high affinity for all 7 mammalian isoforms of 14-3-3 PMID 10493820. Commercial source of peptide [Enzo #BML-P214-0001 http://www.enzolifesciences.com/BML-P214/r18-peptide/].

Difopein is a construct with two R18 monomers in a single 62 amino acid peptide  PMID 11577088. Commercial source of peptide: [Tocris #1245 http://www.tocris.com/dispprod.php?ItemId=110673]

Difopein is a construct with two R18 monomers in a single 62 amino acid peptide  PMID 11577088. Commercial source of peptide: [Tocris #1245 https://www.tocris.com/dispprod.php?ItemId=110673]

==YFP==

==YFP==

YFP is 239 amino acids long (717 bp) according to plasmid map of Invitrogen [http://www.lifetechnologies.com/order/catalog/product/V35820 Vivid ColorsTM pcDNATM/6.2 N-terminal EmGFP/YFP-DEST vectors] see p, 28 of the manual. Difopein is 62 amino acids long (186 bp), so total YFP-difopein fusion protein should be ~ 301 amino acids (903 bp).

YFP is 239 amino acids long (717 bp) according to plasmid map of Invitrogen [http://www.thermofisher.com/order/catalog/product/V35820 Vivid ColorsTM pcDNATM/6.2 N-terminal EmGFP/YFP-DEST vectors] see p, 28 of the manual. Difopein is 62 amino acids long (186 bp), so total YFP-difopein fusion protein should be ~ 301 amino acids (903 bp).

== 14-3-3 FKO papers==

== 14-3-3 FKO papers==