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DMH Sodium Butyrate and Saccharin CTA Controls (view source)
Revision as of 14:12, 27 January 2021
, 14:12, 27 January 2021added agenda
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Replication (to increase N) of [[DMF Sodium Butyrate and Saccharin-LiCl CTA]] and [[DMG Sodium Butyrate and Saccharin-LiCl CTA]] with NaCl-conditioned control groups.
+Test if systemic Sodium Butyrate (NaB), an HDAC inhibitor, enhances CTA learning .
+
+A more strict replication of [https://houptlab.org/papers/pdfs/Houpt065.pdf Kwon and Houpt, Brain Research, 1333 (2010) 36–47], PMID: 20346924 [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2871962/ PMCID: PMC2871962].
+
+1. Sodium Butyrate ([https://www.sigmaaldrich.com/catalog/search?term=303410 Sigma-Aldrich 303410-100G] Butyric acid sodium salt, 98% ).
+
+2. n=8 male, n=8 female, counterbalanced
+
+
+Dose of NaB based on [https://houptlab.org/papers/pdfs/Houpt065.pdf Kwon and Houpt, Brain Research, 1333 (2010) 36–47], PMID: 20346924 [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2871962/ PMCID: PMC2871962]
+
+* 0.3 M Na Butyrate = 3.3g/100ml water
+* 0.3 M NaCl = 18g/liter water (osmotic control)
+
+<br>
+
+==Data==
+
+'''[https://houptlab.org/bartab/expt/?id=DMH Raw Data Link]'''
+
+==People==
+
+Durba Mukherjee
+
+
+==CTA Conditioning==
+
+===Agenda===
+
+<br>
+
+'''Rats arrive''' (January 12, 2021)
+n=16 adult male (8) and female(8) sprague-dawley rats (Charles River CD), individually housed in BRF 169.
+
+<br>
+
+'''Day 0: Water removed''' (January 19, 2021)
+
+<br>
+
+'''Days 1 - 7: Water Restriction''' (January 20 - January 26, 2021)
+
+Weigh rats daily. water for 10 min, ad lib food.
+
+10 min water at 1430 daily.
+
+<br>
+
+'''Day 8: Conditioning Day''' (January 27 2021)
+
+0 min: 30 min access to 0.125% saccharin (5 g sodium saccharin/4L water)
+30 min: all rats injected with 0.15 M NaCl, 3 ml/kg ip.
+40 min: half of rats (1-4, 9-12) injected with 0.3 M NaCl 12 ml/kg ip; other half of rats (5-8, 13-16) injected with 0.3 M Na Butyrate 12 ml/kg, ip
+
+ad lib water returned after final injection overnight.
+
+* Rats 1-8 : female, Rats 9-16: male
+* All rats: saccharin
+* All rats: NaCl (0.15M, 3ml/kg)
+* rats 1-4, 9-12: NaCL injection; rats 5-8,13-16: NaB injection;
+
+
+'''Days 9 - ??: Preference Tests''' (January 28 - ?? February , 2021)
+
+Rats CTA acquisition and extinction was measured with 48-h, 2-bottle preference tests. Rats were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for ??? days.
+
+
+[[Category:Flavor Preference]]
+[[Category:Taste Aversion]]