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DMI Sodium Butyrate and Saccharin CTA Controls 2 (view source)
Revision as of 14:38, 18 February 2021
, 14:38, 18 February 2021created page
Replication (to increase N) of [[DMF Sodium Butyrate and Saccharin-LiCl CTA]] and [[DMG Sodium Butyrate and Saccharin-LiCl CTA]] and [[DMH Sodium Butyrate and Saccharin CTA Controls]] with NaCl-conditioned control groups.
Test if systemic Sodium Butyrate (NaB), an HDAC inhibitor, enhances CTA learning .
A more strict replication of [https://houptlab.org/papers/pdfs/Houpt065.pdf Kwon and Houpt, Brain Research, 1333 (2010) 36–47], PMID: 20346924 [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2871962/ PMCID: PMC2871962].
1. Sodium Butyrate ([https://www.sigmaaldrich.com/catalog/search?term=303410 Sigma-Aldrich 303410-100G] Butyric acid sodium salt, 98% ).
2. n=8 male, n=10 female, counterbalanced (female rat # increased to replace rats that dropped out of DMH)
Dose of NaB based on [https://houptlab.org/papers/pdfs/Houpt065.pdf Kwon and Houpt, Brain Research, 1333 (2010) 36–47], PMID: 20346924 [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2871962/ PMCID: PMC2871962]
* 0.3 M Na Butyrate = 3.3g/100ml water
* 0.3 M NaCl = 18g/liter water (osmotic control)
<br>
==Data==
'''[https://houptlab.org/bartab/expt/?id=DMI Raw Data Link]'''
==People==
Durba Mukherjee
==CTA Conditioning==
===Agenda===
<br>
'''Rats arrive''' (Febryart 2, 2021)
n=16 adult male (8) and female(10) sprague-dawley rats (Charles River CD), individually housed in BRF 169.
<br>
'''Day 0: Water removed''' (February 9, 2021)
<br>
'''Days 1 - 7: Water Restriction''' (February 10 - February 17, 2021)
Weigh rats daily. water for 10 min, ad lib food.
10 min water at 1430 daily.
<br>
'''Day 8: Conditioning Day''' (February 18 2021)
0 min: 30 min access to 0.125% saccharin (5 g sodium saccharin/4L water)
30 min: all rats injected with 0.15 M NaCl, 3 ml/kg ip.
40 min: half of rats (1-4, 9-12) injected with 0.3 M NaCl 12 ml/kg ip; other half of rats (5-8, 13-16) injected with 0.3 M Na Butyrate 12 ml/kg, ip
ad lib water returned after final injection overnight.
* Rats 1-8 : male, Rats 9-18: female
* All rats: saccharin
* All rats: NaCl (0.15M, 3ml/kg)
* rats 1-4, 9-13: NaCl injection; rats 5-8,14-18: NaB injection;
'''Days 9 - ??: Preference Tests''' (February 19 - ?? March , 2021)
Rats CTA acquisition and extinction was measured with 48-h, 2-bottle preference tests. Rats were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for ??? days.
[[Category:Flavor Preference]]
[[Category:Taste Aversion]]
Test if systemic Sodium Butyrate (NaB), an HDAC inhibitor, enhances CTA learning .
A more strict replication of [https://houptlab.org/papers/pdfs/Houpt065.pdf Kwon and Houpt, Brain Research, 1333 (2010) 36–47], PMID: 20346924 [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2871962/ PMCID: PMC2871962].
1. Sodium Butyrate ([https://www.sigmaaldrich.com/catalog/search?term=303410 Sigma-Aldrich 303410-100G] Butyric acid sodium salt, 98% ).
2. n=8 male, n=10 female, counterbalanced (female rat # increased to replace rats that dropped out of DMH)
Dose of NaB based on [https://houptlab.org/papers/pdfs/Houpt065.pdf Kwon and Houpt, Brain Research, 1333 (2010) 36–47], PMID: 20346924 [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2871962/ PMCID: PMC2871962]
* 0.3 M Na Butyrate = 3.3g/100ml water
* 0.3 M NaCl = 18g/liter water (osmotic control)
<br>
==Data==
'''[https://houptlab.org/bartab/expt/?id=DMI Raw Data Link]'''
==People==
Durba Mukherjee
==CTA Conditioning==
===Agenda===
<br>
'''Rats arrive''' (Febryart 2, 2021)
n=16 adult male (8) and female(10) sprague-dawley rats (Charles River CD), individually housed in BRF 169.
<br>
'''Day 0: Water removed''' (February 9, 2021)
<br>
'''Days 1 - 7: Water Restriction''' (February 10 - February 17, 2021)
Weigh rats daily. water for 10 min, ad lib food.
10 min water at 1430 daily.
<br>
'''Day 8: Conditioning Day''' (February 18 2021)
0 min: 30 min access to 0.125% saccharin (5 g sodium saccharin/4L water)
30 min: all rats injected with 0.15 M NaCl, 3 ml/kg ip.
40 min: half of rats (1-4, 9-12) injected with 0.3 M NaCl 12 ml/kg ip; other half of rats (5-8, 13-16) injected with 0.3 M Na Butyrate 12 ml/kg, ip
ad lib water returned after final injection overnight.
* Rats 1-8 : male, Rats 9-18: female
* All rats: saccharin
* All rats: NaCl (0.15M, 3ml/kg)
* rats 1-4, 9-13: NaCl injection; rats 5-8,14-18: NaB injection;
'''Days 9 - ??: Preference Tests''' (February 19 - ?? March , 2021)
Rats CTA acquisition and extinction was measured with 48-h, 2-bottle preference tests. Rats were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for ??? days.
[[Category:Flavor Preference]]
[[Category:Taste Aversion]]