1,093
edits
Changes
Jump to navigation
Jump to search
Test if pre-conditioning injection of sodium valproate enhances conditioned taste aversion induced by low dose of LiCl in females vs. male rats
==Data==
[https://houptlab.org/bartab/expt/?id=DMJ Raw Data Link]
==People==
Durba Mukherjee
==CTA Conditioning==
===Agenda===
'''Rats arrive''' (March 16, 2021)
n=16 adult male (8) and female(10) sprague-dawley rats (Charles River CD), individually housed in BRF 169.
<br>
'''Day 0: Water removed''' (March 22, 2021)
<br>
'''Days 1 - 10: Water Restriction''' (March 23 - April 2, 2021)
Weigh rats daily. Water for 30 min, ad lib food.
30 min water at 1430 daily.
<br>
'''Day 11: Conditioning Day''' (April 3 2021)
-15 min: half of rats injected with 0.15M Na Valproate 8m/kg = 200ml/kg; other half 0.15M NaCl 8ml/kg
0 min: 30 min access to 0.125% saccharin (5 g sodium saccharin/4L water)
30 min: all rats injected with 0.15 M NaCl, 3 ml/kg ip.
40 min: half of rats injected with 0.15 M LiCl 3mlkg ip; other half of rats 0.15 M NaCl 3 ml/kg ip.
ad lib water returned after final injection overnight.
* rats 1-8 : female, Rats 9-18: male
* rats 1-4, 9-12: Valproate; rats 5-8,13-16 NaCl
* All rats: saccharin
* odd rats: 3ml/kg of 0.15M LiCl; odd rats: 3ml/kg of 0.15M NaCl
'''Days 12 - ??: Preference Tests''' (April 4 - ?? March , 2021)
Rats CTA acquisition and extinction was measured with 48-h, 2-bottle preference tests. Rats were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for ??? days.
[[Category:Flavor Preference]]
[[Category:Taste Aversion]]
DMJ Valproate and Saccharin CTA (view source)
Revision as of 13:50, 3 April 2021
, 13:50, 3 April 2021initial page
Test if pre-conditioning injection of sodium valproate enhances conditioned taste aversion induced by low dose of LiCl in females vs. male rats
==Data==
[https://houptlab.org/bartab/expt/?id=DMJ Raw Data Link]
==People==
Durba Mukherjee
==CTA Conditioning==
===Agenda===
'''Rats arrive''' (March 16, 2021)
n=16 adult male (8) and female(10) sprague-dawley rats (Charles River CD), individually housed in BRF 169.
<br>
'''Day 0: Water removed''' (March 22, 2021)
<br>
'''Days 1 - 10: Water Restriction''' (March 23 - April 2, 2021)
Weigh rats daily. Water for 30 min, ad lib food.
30 min water at 1430 daily.
<br>
'''Day 11: Conditioning Day''' (April 3 2021)
-15 min: half of rats injected with 0.15M Na Valproate 8m/kg = 200ml/kg; other half 0.15M NaCl 8ml/kg
0 min: 30 min access to 0.125% saccharin (5 g sodium saccharin/4L water)
30 min: all rats injected with 0.15 M NaCl, 3 ml/kg ip.
40 min: half of rats injected with 0.15 M LiCl 3mlkg ip; other half of rats 0.15 M NaCl 3 ml/kg ip.
ad lib water returned after final injection overnight.
* rats 1-8 : female, Rats 9-18: male
* rats 1-4, 9-12: Valproate; rats 5-8,13-16 NaCl
* All rats: saccharin
* odd rats: 3ml/kg of 0.15M LiCl; odd rats: 3ml/kg of 0.15M NaCl
'''Days 12 - ??: Preference Tests''' (April 4 - ?? March , 2021)
Rats CTA acquisition and extinction was measured with 48-h, 2-bottle preference tests. Rats were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for ??? days.
[[Category:Flavor Preference]]
[[Category:Taste Aversion]]