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DMJ Valproate and Saccharin CTA (view source)
Revision as of 12:10, 26 August 2021
, 12:10, 26 August 2021add valporate solution instructions
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Some evidence that valproate can induce CTA when administered after tastant intake, so in this experiment valproate administered 15 min before the start of saccharin consumption in the hopes that any backward conditioning will be weak. Valproate also has a longer half-life than butyrate, so may be in the system longer.
Some evidence that valproate can induce CTA when administered after tastant intake, so in this experiment valproate administered 15 min before the start of saccharin consumption in the hopes that any backward conditioning will be weak. Valproate also has a longer half-life than butyrate, so may be in the system longer.
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+Sodium Valproate Solution:
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+To maintain osmolarity of 300 mOsm, mix 0.15 M in sterile water.
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+0.15M NaValporate = 24.9 g / l = 1.25 g / 50 ml = 25 mg/ml
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+8 ml/kg = 200 mg/kg
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'''Day 11: Conditioning Day''' (April 3 2021)
'''Day 11: Conditioning Day''' (April 3 2021)
−-15 min: half of rats injected with 0.15M Na Valproate 8m/kg = 200ml/kg; other half 0.15M NaCl 8ml/kg
+-15 min: half of rats injected with 0.15M Na Valproate 8ml/kg = 200mg/kg; other half 0.15M NaCl 8ml/kg
0 min: 30 min access to 0.125% saccharin (5 g sodium saccharin/4L water)
0 min: 30 min access to 0.125% saccharin (5 g sodium saccharin/4L water)
30 min: all rats injected with 0.15 M NaCl, 3 ml/kg ip.
30 min: all rats injected with 0.15 M NaCl, 3 ml/kg ip.