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DML Lower Valproate and Saccharin CTA (view source)
Revision as of 12:25, 26 August 2021
, 12:25, 26 August 2021added agenda outline
Line 9:
Line 9:
8 ml/kg = 20 mg/kg
8 ml/kg = 20 mg/kg
+
+
+
+==Data==
+
+[https://houptlab.org/bartab/expt/?id=DML Raw Data Link]
+
+==People==
+
+Durba Mukherjee
+
+==CTA Conditioning==
+
+===Agenda===
+
+'''Rats arrive''' (August 10, 2021)
+n=16 adult male (8) and female(8) sprague-dawley rats (Charles River CD), individually housed in BRF 169.
+
+<br>
+
+'''Day 0: Water removed''' ( August 17, 2021)
+
+<br>
+
+'''Days 1 - 8: Water Restriction''' (August 18 - 25, 2021)
+
+Weigh rats daily. Water for 30 min, ad lib food.
+
+30 min water at 1430 daily.
+
+<br>
+
+'''Day 9: Conditioning Day''' (August 26 2021)
+
+-15 min: half of rats injected with 15mM Na Valproate 8ml/kg = 20mg/kg; other half 0.15M NaCl 8ml/kg
+0 min: 30 min access to 0.125% saccharin (5 g sodium saccharin/4L water)
+30 min: all rats injected with 0.15 M NaCl, 3 ml/kg ip.
+40 min: half of rats injected with 0.15 M LiCl 3mlkg ip; other half of rats 0.15 M NaCl 3 ml/kg ip.
+
+ad lib water returned after final injection overnight.
+
+* rats 1-8 : female, Rats 9-16: male
+* rats 1-4, 9-12: Valproate; rats 5-8,13-16 NaCl
+* All rats: saccharin
+* odd rats: 3ml/kg of 0.15M LiCl; odd rats: 3ml/kg of 0.15M NaCl
+
+
+'''Days 12 - ???: Preference Tests''' (August 27 - ??? , 2021)
+
+Rats CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests. Rats were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 12 days.
+
+
+[[Category:Flavor Preference]]
+[[Category:Taste Aversion]]