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Sodium valproate (C<sub>8</sub>H<sub>15</sub>NaO<sub>2</sub> ) is MW 166.19 .
Because it is a small molecule sodium salt, we prepare sodium valproate in sterile water at 0.15 M (ie 300 mOsm).
0.15M = 25 mg/ml = 1.25 g/ 50 ml sterile H<sub>2</sub>O
Subsequent dilutions were made with sterile isotonic saline (0.15M NaCl) to preserve tonicity at 300 mOsm.
For experiments to determine effects of valproate on conditioned taste aversion, 3 concentrations were tested:
* 200 mg/kg = 8 ml/kg of 150 mM sodium valproate
* 60 mg/kg = 8 ml of 45 mM sodium valproate (15 ml of 150 mM Na Valproate + 35 ml of 150 mM NaCl)
* 20 mg/kg = 8 ml of 15 mM sodium valproate (5 ml of 150 mM Na Valproate + 45 ml of 150 mM NaCl)
(NB: 60 mg/kg is 1/2 log unit between 20 and 200 mg/kg).
Sodium Valproate Dilutions (view source)
Revision as of 14:18, 13 October 2021
, 14:18, 13 October 2021created page
Sodium valproate (C<sub>8</sub>H<sub>15</sub>NaO<sub>2</sub> ) is MW 166.19 .
Because it is a small molecule sodium salt, we prepare sodium valproate in sterile water at 0.15 M (ie 300 mOsm).
0.15M = 25 mg/ml = 1.25 g/ 50 ml sterile H<sub>2</sub>O
Subsequent dilutions were made with sterile isotonic saline (0.15M NaCl) to preserve tonicity at 300 mOsm.
For experiments to determine effects of valproate on conditioned taste aversion, 3 concentrations were tested:
* 200 mg/kg = 8 ml/kg of 150 mM sodium valproate
* 60 mg/kg = 8 ml of 45 mM sodium valproate (15 ml of 150 mM Na Valproate + 35 ml of 150 mM NaCl)
* 20 mg/kg = 8 ml of 15 mM sodium valproate (5 ml of 150 mM Na Valproate + 45 ml of 150 mM NaCl)
(NB: 60 mg/kg is 1/2 log unit between 20 and 200 mg/kg).