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Line 2:
Line 2: + + + + + + + + + + + + + + + + + + + + + + + + + − + − + + + + + + + + + − Female Sprague Dawley rats (dob 2021-06-07), arrived 2021-08-24.+ − Water removed 2021-11-08+ − Restriction 2021-11-9 thru 2021-11-15+ − + Line 23:
Line 56: − Water returned.+ + + + + + + + − Two-Bottle Test Days: 2021-11-17 to ???+
DMP Ovariectomy and Butyrate-Enhanced CTA (view source)
Revision as of 15:16, 16 November 2021
, 15:16, 16 November 2021added more details, formatting
Test of role of ovarian hormones in butyrate-enhanced CTA.
Test of role of ovarian hormones in butyrate-enhanced CTA.
Dose of NaB based on [https://houptlab.org/papers/pdfs/Houpt065.pdf Kwon and Houpt, Brain Research, 1333 (2010) 36–47], PMID: 20346924 [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2871962/ PMCID: PMC2871962]
* 0.3 M Na Butyrate = 3.3g/100ml water
* 0.3 M NaCl = 18g/liter water (osmotic control)
<br>
==Data==
'''[https://houptlab.org/bartab/expt/?id=DMP Raw Data Link]'''
==People==
Durba Mukherjee
===Agenda===
'''Rats arrive''' (2021-08-24)
Female Sprague Dawley rats n=18 (dob 2021-06-07), arrived
<br>
'''Surgery'''
3 groups: sham surgery, ovariectomized (ovx), ovariectomized + 3mg pellet of 17-beta-estradiol (Innovative Research, Sarasota, FL)
3 groups: sham surgery, ovariectomized (ovx), ovariectomized + 3mg pellet of 17-beta-estradiol (Innovative Research, Sarasota, FL)
DMP1-9: Surgery on 2021-10-24
* DMP1-9: Surgery on 2021-10-24
DMP10-18: Surgery on 2021-10-30
* DMP10-18: Surgery on 2021-10-30
Carpofen 0.5 mg/kg pre-op, isoflurane and bupivicaine intra-op, carpofen 0.5 mg/kg post-op (next day). Staples removed 7 days post-op.
'''Day 0: Water removed 2021-11-08'''
<br>
'''Days 1-7: Water Restriction 2021-11-9 thru 2021-11-15'''
Weigh rats daily. Water for 10 min, ad lib food.
10 min water at 1400 daily.
<br>
Conditioning Day 2021-11-16
'''Day 8: Conditioning Day 2021-11-16'''
* 0-30 min: saccharin 0.125%
* 0-30 min: saccharin 0.125%
* at 40 min: 12 ml/kg of 0.3M NaButyrate or NaCl
* at 40 min: 12 ml/kg of 0.3M NaButyrate or NaCl
ad lib water returned after final injection overnight.
<br>
'''Days 9- ???: Preference Tests: 2021-11-17 to ???'''
Rats CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests. Rats were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 10 days.
[[Category:Taste Aversion]]