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== Phenotyping ==
A swim test was used to phenotype ''het'' mice, ''tlt'' mice, and their littermates. Wiltype mice (+/+) swam toward the side of the pool. Homozygous ''het'' mice (''het''/''het'') and ''tlt'' mice (''tlt''/''tlt'') were identified by their inability to swim while keeping their head above water; instead, they swim in circles and somersault towards underwater. Heterozygotes (''het''/+ or ''tlt''/+) were identified by an intermediate phenotype.
== ''het'' ==
spontaneous mutation B6 x STOCK Nox3<sup>het</sup>/J, Common name: head-tilt
[https://www.jax.org/strain/000061 Strain #000061]
[https://www.jax.org/Protocol?stockNumber=000061&protocolID=38414 PCR protocol]
== ''tlt'' ==
B6.Cg-Otop1<sup>tlt</sup>/J, Common Name: tilted
[https://www.jax.org/strain/001104 Strain #001104]
[https://www.jax.org/Protocol?stockNumber=001104&protocolID=28738 Sequencing protocol]
Vestibular Mutant Mice (view source)
Revision as of 15:29, 17 December 2021
, 15:29, 17 December 2021created page
== Phenotyping ==
A swim test was used to phenotype ''het'' mice, ''tlt'' mice, and their littermates. Wiltype mice (+/+) swam toward the side of the pool. Homozygous ''het'' mice (''het''/''het'') and ''tlt'' mice (''tlt''/''tlt'') were identified by their inability to swim while keeping their head above water; instead, they swim in circles and somersault towards underwater. Heterozygotes (''het''/+ or ''tlt''/+) were identified by an intermediate phenotype.
== ''het'' ==
spontaneous mutation B6 x STOCK Nox3<sup>het</sup>/J, Common name: head-tilt
[https://www.jax.org/strain/000061 Strain #000061]
[https://www.jax.org/Protocol?stockNumber=000061&protocolID=38414 PCR protocol]
== ''tlt'' ==
B6.Cg-Otop1<sup>tlt</sup>/J, Common Name: tilted
[https://www.jax.org/strain/001104 Strain #001104]
[https://www.jax.org/Protocol?stockNumber=001104&protocolID=28738 Sequencing protocol]