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Test of role of ovarian hormones in butyrate-enhanced CTA. Continuation of [[DMP Ovariectomy and Butyrate-Enhanced CTA]].
Dose of NaB based on [https://houptlab.org/papers/pdfs/Houpt065.pdf Kwon and Houpt, Brain Research, 1333 (2010) 36–47], PMID: 20346924 [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2871962/ PMCID: PMC2871962]
* 0.3 M Na Butyrate = 3.3g/100ml water
* 0.3 M NaCl = 18g/liter water (osmotic control)
<br>
==Data==
'''[https://houptlab.org/bartab/expt/?id=DMR Raw Data Link]'''
==People==
Durba Mukherjee
===Agenda===
'''Rats arrive''' (?????)
Female Sprague Dawley rats n=13 (dob ????? ), arrived
<br>
'''Surgery'''
3 groups: sham surgery, ovariectomized (ovx), ovariectomized + 3mg pellet of 17-beta-estradiol (Innovative Research, Sarasota, FL)
* DMR ??? : Surgery on ????
* DMR ??? : Surgery on ????
* DMR ???: Surgery on ????
Carpofen 0.5 mg/kg pre-op, isoflurane and bupivicaine intra-op, carpofen 0.5 mg/kg post-op (next day). Staples removed 7 days post-op.
'''Day 0: Water removed 2022-1-09'''
<br>
'''Days 1-7: Water Restriction 2022-1-9 thru 2022-1-17'''
Weigh rats daily. Water for 10 min, ad lib food.
10 min water at 1400 daily.
<br>
'''Day 8: Conditioning Day 2022-1-18'''
* 0-30 min: saccharin 0.125%
* at 30 min: 3 ml/kg of 0.15M LiCl ip (all rats)
* at 40 min: 12 ml/kg of 0.3M NaButyrate or NaCl
ad lib water returned after final injection overnight.
[PUT GROUP ASSIGNMENTS HERE]
<br>
'''Days 9 - 23: Preference Tests: 2022-1-19 to ????'''
Rats CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests (for ???? days). Rats were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 10 days.
[[Category:Taste Aversion]]
[[Category: CTA Experiments]]
DMR Ovariectomy and Butyrate-Enhanced CTA 2 (view source)
Revision as of 15:06, 19 January 2022
, 15:06, 19 January 2022created page
Test of role of ovarian hormones in butyrate-enhanced CTA. Continuation of [[DMP Ovariectomy and Butyrate-Enhanced CTA]].
Dose of NaB based on [https://houptlab.org/papers/pdfs/Houpt065.pdf Kwon and Houpt, Brain Research, 1333 (2010) 36–47], PMID: 20346924 [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2871962/ PMCID: PMC2871962]
* 0.3 M Na Butyrate = 3.3g/100ml water
* 0.3 M NaCl = 18g/liter water (osmotic control)
<br>
==Data==
'''[https://houptlab.org/bartab/expt/?id=DMR Raw Data Link]'''
==People==
Durba Mukherjee
===Agenda===
'''Rats arrive''' (?????)
Female Sprague Dawley rats n=13 (dob ????? ), arrived
<br>
'''Surgery'''
3 groups: sham surgery, ovariectomized (ovx), ovariectomized + 3mg pellet of 17-beta-estradiol (Innovative Research, Sarasota, FL)
* DMR ??? : Surgery on ????
* DMR ??? : Surgery on ????
* DMR ???: Surgery on ????
Carpofen 0.5 mg/kg pre-op, isoflurane and bupivicaine intra-op, carpofen 0.5 mg/kg post-op (next day). Staples removed 7 days post-op.
'''Day 0: Water removed 2022-1-09'''
<br>
'''Days 1-7: Water Restriction 2022-1-9 thru 2022-1-17'''
Weigh rats daily. Water for 10 min, ad lib food.
10 min water at 1400 daily.
<br>
'''Day 8: Conditioning Day 2022-1-18'''
* 0-30 min: saccharin 0.125%
* at 30 min: 3 ml/kg of 0.15M LiCl ip (all rats)
* at 40 min: 12 ml/kg of 0.3M NaButyrate or NaCl
ad lib water returned after final injection overnight.
[PUT GROUP ASSIGNMENTS HERE]
<br>
'''Days 9 - 23: Preference Tests: 2022-1-19 to ????'''
Rats CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests (for ???? days). Rats were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 10 days.
[[Category:Taste Aversion]]
[[Category: CTA Experiments]]