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DMT Ovariectomy and Butyrate-Enhanced CTA 3 (view source)
Revision as of 17:12, 3 March 2022
, 17:12, 3 March 2022create page
Test of role of ovarian hormones in butyrate-enhanced CTA. Continuation of [[DMP Ovariectomy and Butyrate-Enhanced CTA]].
Dose of NaB based on [https://houptlab.org/papers/pdfs/Houpt065.pdf Kwon and Houpt, Brain Research, 1333 (2010) 36–47], PMID: 20346924 [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2871962/ PMCID: PMC2871962]
* 0.3 M Na Butyrate = 3.3g/100ml water
* 0.3 M NaCl = 18g/liter water (osmotic control)
<br>
==Data==
'''[https://houptlab.org/bartab/expt/?id=DMT Raw Data Link]'''
==People==
Durba Mukherjee
==Agenda==
'''Rats arrive''' (?????)
Female Sprague Dawley rats n=8 (dob ????? ), arrived
<br>
'''Surgery'''
all rats: ovariectomized + 3mg pellet of 17-beta-estradiol (Innovative Research, Sarasota, FL)
* DMT 1-4 : Surgery on ????
* DMT 5-8 : Surgery on ????
Carpofen 0.5 mg/kg pre-op, isoflurane and bupivicaine intra-op, carpofen 0.5 mg/kg post-op (next day). Staples removed 7 days post-op.
'''Day 0: Water removed 2022-1-27'''
<br>
'''Days 1-8: Water Restriction 2022-1-28 thru 2022-2-4'''
Weigh rats daily. Water for 10 min, ad lib food.
10 min water at 1400 daily.
<br>
'''Day 9: Conditioning Day 2022-2-8'''
* 0-30 min: saccharin 0.125%
* at 30 min: 3 ml/kg of 0.15M LiCl ip (all rats)
* at 40 min: 12 ml/kg of 0.3M NaButyrate or NaCl
ad lib water returned after final injection overnight.
Groups:
* OVX + E + LiCl + NaCl: DMT 2,3,4,6
* OVX + E + LiCl + Butyrate: DMT 1,5,7,8
<br>
'''Days 10 - 19: Preference Tests: 2022-2-7 to 2022-2-16'''
Rats CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests (for ???? days). Rats were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 10 days.
[[Category:Taste Aversion]]
[[Category: CTA Experiments]]
Dose of NaB based on [https://houptlab.org/papers/pdfs/Houpt065.pdf Kwon and Houpt, Brain Research, 1333 (2010) 36–47], PMID: 20346924 [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2871962/ PMCID: PMC2871962]
* 0.3 M Na Butyrate = 3.3g/100ml water
* 0.3 M NaCl = 18g/liter water (osmotic control)
<br>
==Data==
'''[https://houptlab.org/bartab/expt/?id=DMT Raw Data Link]'''
==People==
Durba Mukherjee
==Agenda==
'''Rats arrive''' (?????)
Female Sprague Dawley rats n=8 (dob ????? ), arrived
<br>
'''Surgery'''
all rats: ovariectomized + 3mg pellet of 17-beta-estradiol (Innovative Research, Sarasota, FL)
* DMT 1-4 : Surgery on ????
* DMT 5-8 : Surgery on ????
Carpofen 0.5 mg/kg pre-op, isoflurane and bupivicaine intra-op, carpofen 0.5 mg/kg post-op (next day). Staples removed 7 days post-op.
'''Day 0: Water removed 2022-1-27'''
<br>
'''Days 1-8: Water Restriction 2022-1-28 thru 2022-2-4'''
Weigh rats daily. Water for 10 min, ad lib food.
10 min water at 1400 daily.
<br>
'''Day 9: Conditioning Day 2022-2-8'''
* 0-30 min: saccharin 0.125%
* at 30 min: 3 ml/kg of 0.15M LiCl ip (all rats)
* at 40 min: 12 ml/kg of 0.3M NaButyrate or NaCl
ad lib water returned after final injection overnight.
Groups:
* OVX + E + LiCl + NaCl: DMT 2,3,4,6
* OVX + E + LiCl + Butyrate: DMT 1,5,7,8
<br>
'''Days 10 - 19: Preference Tests: 2022-2-7 to 2022-2-16'''
Rats CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests (for ???? days). Rats were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 10 days.
[[Category:Taste Aversion]]
[[Category: CTA Experiments]]