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Fluorescent Antibody Dilutions
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Revision as of 15:34, 4 July 2022
40 bytes added
,
15:34, 4 July 2022
Fix table to use tabs.
Line 51:
Line 51:
==Layout of Immuno Wells==
==Layout of Immuno Wells==
−
<tab>
+
<
tab sep=
tab>
Seconday Dilution (1:x)
Seconday Dilution (1:x)
Primary Dilution (1:x) ∞ 500 200 100 boiled 1:100 Total 1°
Primary Dilution (1:x) ∞ 500 200 100 boiled 1:100 Total 1°
Line 74:
Line 74:
'''Volume of Primary stock (1:100) to add for each anti Fos and anti pCREB:'''
'''Volume of Primary stock (1:100) to add for each anti Fos and anti pCREB:'''
−
<tab>
+
<
tab sep=
tab>
Seconday Dilution (1:x)
Seconday Dilution (1:x)
Primary Dilution (1:x) ∞ 500 200 100 boiled 1:100 Total 1°
Primary Dilution (1:x) ∞ 500 200 100 boiled 1:100 Total 1°
Line 88:
Line 88:
'''PBS/BSA to add for Primary:'''
'''PBS/BSA to add for Primary:'''
−
<tab>
+
<
tab sep=
tab>
Seconday Dilution (1:x)
Seconday Dilution (1:x)
Primary Dilution (1:x) ∞ 500 200 100 boiled 1:100 Total 1°
Primary Dilution (1:x) ∞ 500 200 100 boiled 1:100 Total 1°
Line 102:
Line 102:
'''Volume of Secondary Stock (1:100) to add for each anti-rabbit and anti-goat:'''
'''Volume of Secondary Stock (1:100) to add for each anti-rabbit and anti-goat:'''
−
<tab>
+
<
tab sep=
tab>
Seconday Dilution (1:x)
Seconday Dilution (1:x)
Primary Dilution (1:x) ∞ 500 200 100 boiled 1:100
Primary Dilution (1:x) ∞ 500 200 100 boiled 1:100
Line 116:
Line 116:
'''PBS/BSA to add for Secondary:'''
'''PBS/BSA to add for Secondary:'''
−
<tab>
+
<
tab sep=
tab>
Seconday Dilution (1:x)
Seconday Dilution (1:x)
Primary Dilution (1:x) ∞ 500 200 100 boiled 1:100
Primary Dilution (1:x) ∞ 500 200 100 boiled 1:100
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