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added polycose experiment details
JF Rats: Test Selenate Attenuation of Conditioned Taste Aversion (25 days)
JF Rats: Test Selenate Attenuation of Conditioned Taste Aversion (25 days)
Rats: n=12, male, Charles River CD, DOB 2012-11-10, recd 2013-1-15
'''Mon 2013-2-4'''
'''Mon 2013-2-4'''
'''Wed 2013-2-13 ''' Conditioning day
'''Wed 2013-2-13 ''' Conditioning day
* 4 groups (n=6 per group) NaCl/NaCl, Na2SeO4/NaCl, NaCl/LiCl, Na2SeO4/LiCl
* 4 groups (n=6 per group)
# NaCl/NaCl (JF01,5,9,13,17,21)
# Na2SeO4/NaCl (JF02,6,10,14,18,22)
# NaCl/LiCl (JF03,7,11,15,19,23)
# Na2SeO4/LiCl (JF04,8,12,16,20,24)
* at - 2 h, inject with Na2SeO4 (0.5 mg/kg i.p.) or NaCl
* at - 2 h, inject with Na2SeO4 (0.5 mg/kg i.p.) or NaCl
* at 0 h, 10 min access to 0.125% saccharin
* at 0 h, 10 min access to 0.125% saccharin
== Polycose retest? ==
== Polycose retest ==
Restart restriction on 2013-3-1 to retest same groups without selenate, using 8% polycose as CS, to determine if still competent to acquire CTA
'''2013-3-1 - 2013-3-4''' Water Restriction
'''2013-3-5''' Conditioning Day
no pretreatment; 8% polycose for 30 min, followed by 12 ml/kg ip 0.15M NaCl (NaCl/NaCl and NaSeO4/NaCl groups) or LiCl (NaCl/LiCl and NaSeO4/LiCl groups)
'''2013-3-6 - 2013-3-11'''
2-bottle, 24-h preference test (8% polycose vs. water) for 6 days.