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DMW Ovariectomy and Butyrate-Enhanced CTA 4 (view source)
Revision as of 08:08, 18 June 2023
, 08:08, 18 June 2023created page
Test of role of ovarian hormones in butyrate-enhanced CTA. Continuation of [[DMP Ovariectomy and Butyrate-Enhanced CTA]].
{{Infobox experiment|align=right
| Name = Ovariectomy and Butyrate-Enhanced CTA 4
| project = HDAC Sex Difference
| code = DMW
| protocol = 202200000006
| funding = Chair's Fund
| investigators = Houpt, Bass, Cote, D. Jorge
| species = rat
| date = 2023-6-18
| type = CTA
| drugs = butyrate, lithium
| artifacts =
| data = https://houptlab.org/bartab/expt/?id=DMW
}}
Dose of NaB based on [https://houptlab.org/papers/pdfs/Houpt065.pdf Kwon and Houpt, Brain Research, 1333 (2010) 36–47], PMID: 20346924 [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2871962/ PMCID: PMC2871962]
* 0.3 M Na Butyrate = 3.3g/100ml water
* 0.3 M NaCl = 18g/liter water (osmotic control)
<br>
==Data==
'''[https://houptlab.org/bartab/expt/?id=DMW Raw Data Link]'''
==People==
Durba Mukherjee
==Agenda==
'''Rats arrive'''
Female Sprague Dawley rats n = XXX, d.o.b. XXXX, arrived: XXXX
<br>
'''Surgery'''
all rats: ovariectomized + 3mg pellet of 17-beta-estradiol (Innovative Research, Sarasota, FL)
* DMW 1-XXX : Surgery on 2023-
Carpofen 0.5 mg/kg pre-op, isoflurane and bupivicaine intra-op, carpofen 0.5 mg/kg post-op (next day). Staples removed 7 days post-op.
'''Day 0: Water removed XXXX''
<br>
'''Days 1-8: Water Restriction XXXX thru XXXX'''
Weigh rats daily. Water for 10 min, ad lib food.
10 min water at 1400 daily.
<br>
'''Day 9: Conditioning Day XXXX'''
* 0-30 min: saccharin 0.125%
* at 30 min: 3 ml/kg of 0.15M LiCl ip (all rats)
* at 40 min: 12 ml/kg of 0.3M NaButyrate or NaCl
ad lib water returned after final injection overnight.
Groups:
* OVX + LiCl + NaCl: DMW XXX
* OVX + LiCl + Butyrate: DMW XXX
* Sham + LiCl + NaCl: DMW XXX
* Sham + LiCl + Butyrate: DMW XXX
<br>
'''Days 10 - 19: Preference Tests: XXXX to XXXX'''
Rats CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests (for ???? days). Rats were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 10 days.
[[Category:Taste Aversion]]
[[Category:CTA Experiments]]
[[Category:Epigenetics]]
{{Infobox experiment|align=right
| Name = Ovariectomy and Butyrate-Enhanced CTA 4
| project = HDAC Sex Difference
| code = DMW
| protocol = 202200000006
| funding = Chair's Fund
| investigators = Houpt, Bass, Cote, D. Jorge
| species = rat
| date = 2023-6-18
| type = CTA
| drugs = butyrate, lithium
| artifacts =
| data = https://houptlab.org/bartab/expt/?id=DMW
}}
Dose of NaB based on [https://houptlab.org/papers/pdfs/Houpt065.pdf Kwon and Houpt, Brain Research, 1333 (2010) 36–47], PMID: 20346924 [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2871962/ PMCID: PMC2871962]
* 0.3 M Na Butyrate = 3.3g/100ml water
* 0.3 M NaCl = 18g/liter water (osmotic control)
<br>
==Data==
'''[https://houptlab.org/bartab/expt/?id=DMW Raw Data Link]'''
==People==
Durba Mukherjee
==Agenda==
'''Rats arrive'''
Female Sprague Dawley rats n = XXX, d.o.b. XXXX, arrived: XXXX
<br>
'''Surgery'''
all rats: ovariectomized + 3mg pellet of 17-beta-estradiol (Innovative Research, Sarasota, FL)
* DMW 1-XXX : Surgery on 2023-
Carpofen 0.5 mg/kg pre-op, isoflurane and bupivicaine intra-op, carpofen 0.5 mg/kg post-op (next day). Staples removed 7 days post-op.
'''Day 0: Water removed XXXX''
<br>
'''Days 1-8: Water Restriction XXXX thru XXXX'''
Weigh rats daily. Water for 10 min, ad lib food.
10 min water at 1400 daily.
<br>
'''Day 9: Conditioning Day XXXX'''
* 0-30 min: saccharin 0.125%
* at 30 min: 3 ml/kg of 0.15M LiCl ip (all rats)
* at 40 min: 12 ml/kg of 0.3M NaButyrate or NaCl
ad lib water returned after final injection overnight.
Groups:
* OVX + LiCl + NaCl: DMW XXX
* OVX + LiCl + Butyrate: DMW XXX
* Sham + LiCl + NaCl: DMW XXX
* Sham + LiCl + Butyrate: DMW XXX
<br>
'''Days 10 - 19: Preference Tests: XXXX to XXXX'''
Rats CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests (for ???? days). Rats were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 10 days.
[[Category:Taste Aversion]]
[[Category:CTA Experiments]]
[[Category:Epigenetics]]