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Arrived 2023-2-6.
 
Arrived 2023-2-6.
   −
'''Day 0: Water removed''' (2023-3-4)
+
'''Day 0: Water removed''' (2023-6-19)
      −
'''Days 1 - 11: Water Restriction''' (2023-3-5 - 2023-3-15)
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'''Days 1 - 11: Water Restriction''' (2023-6-20 - 2023-6-30)
    
Weigh mice daily. Ad lib food. Water access on consecutive days at noon: 3h, 3h, 3h, 1h, 1h, 1h, 30min, 30 min, 10 min, 10 min, 10 min.  
 
Weigh mice daily. Ad lib food. Water access on consecutive days at noon: 3h, 3h, 3h, 1h, 1h, 1h, 30min, 30 min, 10 min, 10 min, 10 min.  
   −
Note: Daylight saving time started March 12, so noon -> 1pm
+
 
    
Mice given water bottle and empty bottle, alternating position.  
 
Mice given water bottle and empty bottle, alternating position.  
   −
In the afternoon (~5pm) , mice will be given a supplemental access to water for 30 min.
+
In the afternoon (~4pm), mice will be given supplemental access to water for 30 min.
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'''Day 12: Conditioning Day''' (2023-3-16)
 
'''Day 12: Conditioning Day''' (2023-3-16)
   −
Mice are given 10-min access to 0.125% saccharin solution, then injected with vehicle (10 ml/kg) or 1, 3 or 10 mg/kg  
+
Mice are given 10-min access to 0.125% saccharin solution, then injected with vehicle (10 ml/kg) or 1, 3 or 30 mg/kg  
     
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