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Created page with "=Background= Sodium Selenate (Na2Se04) activates PP2A (1). CTA and Selenate experiments show that male Sprague Dawley rats given systemic injections of 0.5m..."
=Background=
[[Selenium|Sodium Selenate]] (Na2Se04) activates PP2A (1).
[[CTA and Selenate]] experiments show that male Sprague Dawley rats given systemic injections of 0.5mg/kg sodium selenate two hours before taste aversion conditioning form attenuated taste aversions that extinguish rapidly. Selenate also decreases LiCl-induced phospho-MAP kinase in the NTS and lateral PBN.
This experiment will use LCMS to identify differences in protein phosphorylation in the brain two hours after rats receive 0.5mg/kg selenate vs vehicle.
=Agenda=
07/15/2023: 10 rats (5 males, 5 females) were overdosed with euthasol and rapidly decapitated two hours after receiving 1ml/kg i.p. injections of 0.5mg/kg sodium selenate or vehicle (0.15M NaCl). Brains were dissected and washed in ice-cold PP2A storage buffer (Tris-HCl, pH 7.4, EDTA, EGTA, DTT, Sucrose, N-PER, protease inhibitor tablet [will update to include concentrations]), and frozen at -80*C.
{| class="wikitable"
|+ Experiment schedule
|-
! Subject !! Sex !! Treatment !! Weight !! Injection vol (ml) !! Injection time (PM) !! Time of brain dissection (PM)
|-
| MBJO1 || M || NaCl || 371 || 0.37 || 2:17 || 4:28
|-
| MBJ02 ||F || Sel || 257 || 0.26 || 2:26 || 4:34
|-
| MBJ03 || M || Sel || 348 || 0.35 || 2:33 ||
|-
| MBJ04 || F || NaCl || 250 || 0.25 || 2:40 ||
|-
| MBJ05 || M || NaCl || 346 || 0.35 || 2:46 ||5:00
|-
| MBJ06 || F || Sel || 247 || 0.25 || 2:53 ||
|-
| MBJ07 || M || Sel || 324 || 0.33 || 2:59 ||
|-
| MBJ08 || F || NaCl || 245 || 0.25 || 3:04 ||
|-
| MBJ09 || M || NaCl || 335 || 0.34 || 3:10 ||
|-
| MBJ10 || F || Sel || 263 || 0.26 || 3:16 || 5:33
|}
[[Selenium|Sodium Selenate]] (Na2Se04) activates PP2A (1).
[[CTA and Selenate]] experiments show that male Sprague Dawley rats given systemic injections of 0.5mg/kg sodium selenate two hours before taste aversion conditioning form attenuated taste aversions that extinguish rapidly. Selenate also decreases LiCl-induced phospho-MAP kinase in the NTS and lateral PBN.
This experiment will use LCMS to identify differences in protein phosphorylation in the brain two hours after rats receive 0.5mg/kg selenate vs vehicle.
=Agenda=
07/15/2023: 10 rats (5 males, 5 females) were overdosed with euthasol and rapidly decapitated two hours after receiving 1ml/kg i.p. injections of 0.5mg/kg sodium selenate or vehicle (0.15M NaCl). Brains were dissected and washed in ice-cold PP2A storage buffer (Tris-HCl, pH 7.4, EDTA, EGTA, DTT, Sucrose, N-PER, protease inhibitor tablet [will update to include concentrations]), and frozen at -80*C.
{| class="wikitable"
|+ Experiment schedule
|-
! Subject !! Sex !! Treatment !! Weight !! Injection vol (ml) !! Injection time (PM) !! Time of brain dissection (PM)
|-
| MBJO1 || M || NaCl || 371 || 0.37 || 2:17 || 4:28
|-
| MBJ02 ||F || Sel || 257 || 0.26 || 2:26 || 4:34
|-
| MBJ03 || M || Sel || 348 || 0.35 || 2:33 ||
|-
| MBJ04 || F || NaCl || 250 || 0.25 || 2:40 ||
|-
| MBJ05 || M || NaCl || 346 || 0.35 || 2:46 ||5:00
|-
| MBJ06 || F || Sel || 247 || 0.25 || 2:53 ||
|-
| MBJ07 || M || Sel || 324 || 0.33 || 2:59 ||
|-
| MBJ08 || F || NaCl || 245 || 0.25 || 3:04 ||
|-
| MBJ09 || M || NaCl || 335 || 0.34 || 3:10 ||
|-
| MBJ10 || F || Sel || 263 || 0.26 || 3:16 || 5:33
|}