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Anorexic anx/anx mice [https://www.jax.org/strain/000624 Jackson Lab strain 000624].
Mutation mapped to a 0.2 cM interval residing between the markers D2Mit133 and Jojo5 chromosome 2 (Chr 2: bp 118, 889, 896–120, 175, 1081) (Lindfors et al., 2011).
== Possible genes affected ==
Lindsfor 2011: NADH dehydrogenase (ubiquinone) 1a-subcomplex (Ndufaf1)
Kim 2017: tyrosine kinase receptor Tyro3 which they conclude is not the anx-mutation but a strain specific modifier of anx-phenotypes
== Abnormalities Table ==
== Tyro3 Genotyping ==
TYRO3 protein tyrosine kinase 3 (Tyro3) mutation in anx/anx eliminatees a NlaIV restriction site.
* primers 5′-GATGGCGCTGAGGCGGAGCATG and 5′-CGCGGC- CGGAGGTCTGGCAG ; annealing temperature 72C
* subsequent digestion with NlaIV
* wildtype -> 175 bp fragment, anx/anx -> 250 bp product
Anorexia anx/anx mutant mice (view source)
Revision as of 12:33, 16 January 2024
, 12:33, 16 January 2024created page
Anorexic anx/anx mice [https://www.jax.org/strain/000624 Jackson Lab strain 000624].
Mutation mapped to a 0.2 cM interval residing between the markers D2Mit133 and Jojo5 chromosome 2 (Chr 2: bp 118, 889, 896–120, 175, 1081) (Lindfors et al., 2011).
== Possible genes affected ==
Lindsfor 2011: NADH dehydrogenase (ubiquinone) 1a-subcomplex (Ndufaf1)
Kim 2017: tyrosine kinase receptor Tyro3 which they conclude is not the anx-mutation but a strain specific modifier of anx-phenotypes
== Abnormalities Table ==
== Tyro3 Genotyping ==
TYRO3 protein tyrosine kinase 3 (Tyro3) mutation in anx/anx eliminatees a NlaIV restriction site.
* primers 5′-GATGGCGCTGAGGCGGAGCATG and 5′-CGCGGC- CGGAGGTCTGGCAG ; annealing temperature 72C
* subsequent digestion with NlaIV
* wildtype -> 175 bp fragment, anx/anx -> 250 bp product