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JCF Excitatory DREADD Functional Validation (view source)
Revision as of 16:05, 25 January 2024
, 25 JanuaryCreated page with "To validate FosCreER x DREADD mice by testing if active cellular populations after LiCl exposure can be inhibited with CNO administration to reduce/prevent taste aversion. C..."
To validate FosCreER x DREADD mice by testing if active cellular populations after LiCl exposure can be inhibited with CNO administration to reduce/prevent taste aversion.
CNO ordering information / lot numbers
CNO preparation (e.g. vehicle, how its dissolved)
{{Infobox experiment|align=right
| Name = Clozapine-N-Oxide Taste Aversion
| project = DREADDs and Magentic Field
| code = JCE
| protocol = 202200000006
| funding = Chair's Fund
| investigators = Cote, Houpt
| species = mice
| date = 2023-6-19
| type = CTA
| drugs = NaCl, CNO
| artifacts =
| data = https://houptlab.org/bartab/expt/?id=JCF}
==Data==
'''[https://houptlab.org/bartab/expt/?id=JCF Raw Data Link]'''
==People==
Jason Cote
==Agenda==
Mice In House Colony FosCreER X hM4Di (n=11 male DOB +/-, n=12 female DOB +/-)
Drug Preparation: Isotonic LiCl (0.15M,20ml/kg, i.p) followed by 4-OHT prepared at 5mg/ml (50mg/kg,10ml/kg, i.p) or Sesame oil vehicle (10ml/kg i.p) after 10 min.
Pre Treatment (8/18/23) 2 weeks before starting water restriction
4 groups:
1. Isotonic LiCl and 4-OHT, N=6
2. Isotonic LiCl and sesame oil, N=6
3. Isotonic saline and 4-OHT, N=6
4. Isotonic saline and sesame oil, N=5
'''Day 0: Water removed''' (2023-9-12)
'''Days 1 - 11: Water Restriction''' (2023-9-13 - 2023-9-23)
Weigh mice daily. Ad lib food. Water access on consecutive days at noon: 3h, 3h, 3h, 1h, 1h, 1h, 30min, 30 min, 10 min, 10 min, 10 min.
Mice given water bottle and empty bottle, alternating position.
In the afternoon (~4pm), mice will be given supplemental access to water for 30 min.
'''Day 12: Conditioning Day''' (2023-9-24)
Mice are given 10-min access to 0.125% saccharin solution, then injected with CNO (3mg/kg,10ml/kg, i.p) followed by LiCl (0.15M,20ml/kg, i.p) or isotonic saline after 10 min
Groups:
* LTL: Exp group (predicted to show attenuated taste aversion)
* LVL: + Control (expect to form taste aversion)
* VTV: - Control (expect to not form taste aversion)
* VVV: - Control (expect to not form taste aversion)
* JCE01-04: Female
* JCE05-08: Female
* JCE09-12: Male
* JCE13-16: Male
After injection, ad lib water returned overnight.
'''Day 13 - 24: Preference Tests''' (2023-9-25 - 2023-10-6 )
CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests. Mice were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 7 days.
[[Category:Taste Aversion]]
[[Category:CTA Experiments]]
[[Category:Magnet Experiments]]
CNO ordering information / lot numbers
CNO preparation (e.g. vehicle, how its dissolved)
{{Infobox experiment|align=right
| Name = Clozapine-N-Oxide Taste Aversion
| project = DREADDs and Magentic Field
| code = JCE
| protocol = 202200000006
| funding = Chair's Fund
| investigators = Cote, Houpt
| species = mice
| date = 2023-6-19
| type = CTA
| drugs = NaCl, CNO
| artifacts =
| data = https://houptlab.org/bartab/expt/?id=JCF}
==Data==
'''[https://houptlab.org/bartab/expt/?id=JCF Raw Data Link]'''
==People==
Jason Cote
==Agenda==
Mice In House Colony FosCreER X hM4Di (n=11 male DOB +/-, n=12 female DOB +/-)
Drug Preparation: Isotonic LiCl (0.15M,20ml/kg, i.p) followed by 4-OHT prepared at 5mg/ml (50mg/kg,10ml/kg, i.p) or Sesame oil vehicle (10ml/kg i.p) after 10 min.
Pre Treatment (8/18/23) 2 weeks before starting water restriction
4 groups:
1. Isotonic LiCl and 4-OHT, N=6
2. Isotonic LiCl and sesame oil, N=6
3. Isotonic saline and 4-OHT, N=6
4. Isotonic saline and sesame oil, N=5
'''Day 0: Water removed''' (2023-9-12)
'''Days 1 - 11: Water Restriction''' (2023-9-13 - 2023-9-23)
Weigh mice daily. Ad lib food. Water access on consecutive days at noon: 3h, 3h, 3h, 1h, 1h, 1h, 30min, 30 min, 10 min, 10 min, 10 min.
Mice given water bottle and empty bottle, alternating position.
In the afternoon (~4pm), mice will be given supplemental access to water for 30 min.
'''Day 12: Conditioning Day''' (2023-9-24)
Mice are given 10-min access to 0.125% saccharin solution, then injected with CNO (3mg/kg,10ml/kg, i.p) followed by LiCl (0.15M,20ml/kg, i.p) or isotonic saline after 10 min
Groups:
* LTL: Exp group (predicted to show attenuated taste aversion)
* LVL: + Control (expect to form taste aversion)
* VTV: - Control (expect to not form taste aversion)
* VVV: - Control (expect to not form taste aversion)
* JCE01-04: Female
* JCE05-08: Female
* JCE09-12: Male
* JCE13-16: Male
After injection, ad lib water returned overnight.
'''Day 13 - 24: Preference Tests''' (2023-9-25 - 2023-10-6 )
CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests. Mice were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 7 days.
[[Category:Taste Aversion]]
[[Category:CTA Experiments]]
[[Category:Magnet Experiments]]