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JCF Excitatory DREADD Functional Validation (view source)
Revision as of 15:38, 19 July 2024
, 19 Julyno edit summary
CNO ordering information / lot numbers
CNO ordering information / lot numbers
CNO preparation (e.g. vehicle, how its dissolved)
CNO preparation: Dissolved in isotonic saline 0.3mg/ml shaking
4-OHT preparation: Dissolved in pharmaceutical grade sesame oil 5mg/ml shaking o/n 55c
4-OHT preparation: Dissolved in pharmaceutical grade sesame oil 5mg/ml shaking o/n 55c
==Agenda==
==Agenda==
Mice In House Colony FosCreER X hM3Dq (n=?? male DOB +/-, n=?? female DOB +/-)
Mice In House Colony FosCreER X hM3Dq (n=7 male, n=16 female)
Drug Preparation: Isotonic LiCl (0.15M,20ml/kg, i.p) followed by 4-OHT prepared at 5mg/ml (50mg/kg,10ml/kg, i.p) or Sesame oil vehicle (10ml/kg i.p) after 10 min.
Drug Preparation: Isotonic LiCl (0.15M,20ml/kg, i.p) followed by 4-OHT prepared at 5mg/ml (50mg/kg,10ml/kg, i.p) or Sesame oil vehicle (10ml/kg i.p) after 10 min.