1,114
edits
Changes
Jump to navigation
Jump to search
Anorexia anx/anx mutant mice (view source)
Revision as of 12:07, 29 November 2024
, 12:07, 29 November 2024→PCR clean-up: minor detail
Line 71:
Line 71:
===PCR clean-up===
===PCR clean-up===
−[https://omegabiotek.com/product/pcr-clean-up-kit-e-z-n-a-cycle-pure/ E.Z.N.A. Cycle Pure Kit (V-spin)] yields 30 ul in dH<sub>2</sub>O [ now trying kit elution buffer ]
+[https://omegabiotek.com/product/pcr-clean-up-kit-e-z-n-a-cycle-pure/ E.Z.N.A. Cycle Pure Kit (V-spin)] yields 30 ul in dH<sub>2</sub>O [ because worried about pH of dH<sub>2</sub>O, now trying kit elution buffer which I think is Tris-Cl pH 8.5]
Measure DNA quantity/quality with Nanodrop.
Measure DNA quantity/quality with Nanodrop.