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MNase Digestion
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Revision as of 11:28, 1 June 2025
23 bytes added
,
11:28, 1 June 2025
test Soheila account
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* Separate medulla in PBS
* Separate medulla in PBS
* Homogenized 4 medulla in NIB
* Homogenized 4 medulla in NIB
+
* (filter homogenate?)
* Crosslinking cells with 1% formaldehyde, 15min, RT
* Crosslinking cells with 1% formaldehyde, 15min, RT
* Quenching with 1/20 of stock Glycine (2.5M), final concentration is 125mM, 10min, RT
* Quenching with 1/20 of stock Glycine (2.5M), final concentration is 125mM, 10min, RT
Soheila
2
edits