2
edits
Changes
Jump to navigation
Jump to search
Line 2:
Line 2: +
test Soheila account
* Separate medulla in PBS
* Separate medulla in PBS
* Homogenized 4 medulla in NIB
* Homogenized 4 medulla in NIB
* (filter homogenate?)
* Crosslinking cells with 1% formaldehyde, 15min, RT
* Crosslinking cells with 1% formaldehyde, 15min, RT
* Quenching with 1/20 of stock Glycine (2.5M), final concentration is 125mM, 10min, RT
* Quenching with 1/20 of stock Glycine (2.5M), final concentration is 125mM, 10min, RT