Changes

23 bytes added ,  11:28, 1 June 2025
test Soheila account
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* Separate medulla in PBS
 
* Separate medulla in PBS
 
* Homogenized 4 medulla in NIB  
 
* Homogenized 4 medulla in NIB  
 +
* (filter homogenate?)
 
* Crosslinking cells with 1% formaldehyde, 15min, RT
 
* Crosslinking cells with 1% formaldehyde, 15min, RT
 
* Quenching with 1/20 of stock Glycine (2.5M), final concentration is 125mM, 10min, RT
 
* Quenching with 1/20 of stock Glycine (2.5M), final concentration is 125mM, 10min, RT
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edits