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Line 26: − 0.15M LiOr: mix equal volumes of 0.3 M LiOH and 0.3 M orotic acid+ − + − + − * 0.3M LiOH (MW 23.95) = 7.19 g / liter, 0.14 g / 20 ml − * 0.3M NaOH (MW 39.997) = 12 g / liter, 0.24 g/20ml + + + Line 48:
Line 49: − +
revised LiOr preparation
== LiOr Preparation ==
== LiOr Preparation ==
From Pacholko 2023 PMID 37356352: LiOr was synthesized by combining lithium hydroxide (Sigma-Aldrich), and orotic acid (Sigma-Aldrich) in a 1:1 M ratio in distilled water; the NaCl concentration was adjusted to 0.9% and pH to 7.4.
From Pacholko 2023 PMID 37356352: LiOr was synthesized by combining lithium hydroxide (Sigma-Aldrich), and orotic acid (Sigma-Aldrich) in a 1:1 M ratio in distilled water; the NaCl concentration was adjusted to 0.9% and pH to 7.4. Doses were administered as 0.1 ml/10g BW (10 ml/kg), with the highest dose of 15 mg/kg Li+ => 375 mg/kg LiOr => 230 mM (NB: not sure how they got this much to dissolve in water)
[https://www.sigmaaldrich.com/US/en/product/sigma/o2750 orotic acid, Sigma Aldrich O2750-10G ($62.01)] CAS 65-86-1, MW 156.10
[https://www.sigmaaldrich.com/US/en/product/sigma/o2750 orotic acid, Sigma Aldrich O2750-10G ($62.01)] CAS 65-86-1, MW 156.10
[https://www.sigmaaldrich.com/US/en/product/sigald/545856 lithium hydroxide, powder, reagent grade >= 98%, 545856-100G ($45.45)] CAS 1310-65-2, MW 23.95
[https://www.sigmaaldrich.com/US/en/product/sigald/545856 lithium hydroxide, powder, reagent grade >= 98%, 545856-100G ($45.45)] CAS 1310-65-2, MW 23.95
sodium hydroxide, CAS 1310-73-2, MW 40
0.15M NaOr: mix equal volumes of 0.3 M NaOH and 0.3 M orotic acid
* Make 500 ml of 0.15M LiOH (1.80 g LiOH / 500ml dH<small>2</small>0).
* 0.3M orotic acid (MW 156.10) = 46.83 g / liter, 0.94 g / 20 ml
* Make 500 ml of 0.15M NaOH (3.00 g NaOH / 500ml dH<small>2</small>0).
* Make 20 ml of 0.15M LiOr by adding 0.47 g orotic acid to 20 ml of 0.15M LiOH (need to heat?)
* Make 20 ml of 0.15M NaOr by adding 0.47 g orotic acid to 20 ml of 0.15M NaOH (need to heat?)
LiCO should also dissociate completely (citation needed).
LiCO should also dissociate completely (citation needed).
LiOr, however is more tightly bound complex (citation needed). So we'll mix up LiOr in 0.15 NaCl to preserve osmolarity.
LiOr, however is more tightly bound complex (citation needed). So may need to mix up LiOr in 0.15 NaCl to preserve osmolarity.