Changes

added LiOH
Line 12: Line 12:  
NaCl 58.44 (0.39 Na) 8.8 12 105.19 (41.4 Na)
 
NaCl 58.44 (0.39 Na) 8.8 12 105.19 (41.4 Na)
 
NaOr 178.08 (0.13 Na) 26.71 12 320.54 (41.4 Na)
 
NaOr 178.08 (0.13 Na) 26.71 12 320.54 (41.4 Na)
 +
LiOH 23.95 0.29 3.6 na na na
 
LiCl 42.39 0.16 6.35 12 76.30 12.5
 
LiCl 42.39 0.16 6.35 12 76.30 12.5
 
LiCl 42.39 0.16 6.35 20 127.17 41.6
 
LiCl 42.39 0.16 6.35 20 127.17 41.6
Line 32: Line 33:  
* Make 500 ml of 0.15M NaOH (3.00 g NaOH / 500ml dH<small>2</small>0).
 
* Make 500 ml of 0.15M NaOH (3.00 g NaOH / 500ml dH<small>2</small>0).
   −
* Make 20 ml of 0.15M LiOr by adding 0.47 g orotic acid to 20 ml of 0.15M LiOH, vortex: dissolved after about 2 min of vortexing, store at 4C in foil-wrapped tube.
+
* Make 20 ml of 0.15M LiOr by adding 0.47 g orotic acid to 20 ml of 0.15M LiOH, vortex: partially dissolved after about 2 min of vortexing -- but soon precipiates out
 +
 
 +
* So dissolve in 0.3M TrisAmino...
    
* Make 20 ml of 0.15M NaOr by adding 0.47 g orotic acid to 20 ml of 0.15M NaOH, vortex: did NOT dissolve -- milky white suspension! Left shaking overnight...
 
* Make 20 ml of 0.15M NaOr by adding 0.47 g orotic acid to 20 ml of 0.15M NaOH, vortex: did NOT dissolve -- milky white suspension! Left shaking overnight...