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add some potential test solutions
[https://faculty.smcm.edu/wihatch/courses/436web/436resources/osmoWescore5520.html Wescor Vapro 5520 osmometer]
[https://faculty.smcm.edu/wihatch/courses/436web/436resources/osmoWescore5520.html Wescor Vapro 5520 osmometer]
==Test Solutions==
Make mixtures of LiCl, Li<sub>2</sub>CO<sub>3</sub>, and LiOr and several "physiological" solutions. Measure free Li+ with electrode. For Tris and plasma, also measure osmolality.
===1. Tris===
Tris-LiCl, Tris-LiOH, Tris-LiOH-Orotate (across concentration and pH ranges)
===2. Simulated Gastric Fluid (SGF)===
Li+ electrode cannot function at low pH (~2.0) of SGF, so need to neutralize mixtures with sodium bicarbonate (NaCH0<sub>3</sub>). Assay free Li+ when added before or after neutralization.
Measure at 37° C, across concentration ranges:
SGF-LiCl -> + NaHC0<sub>3</sub>, SGF-Tris-LiOr -> + NaHC0<sub>3</sub>, SGF -> + NaHC0<sub>3</sub> + LiCl, SGF -> + NaHC03 + Tris-LiOr
===3. Rat plasma===
Blood taken from terminal cardiac puncture, heparinized, plasma separated and frozen at -20° C.
Measure at 37° C, across concentration ranges:
plasma-LiCl, plasma-Li<sub>2</sub>CO<sub>3</sub>, plasma-LiOr
===4. Artificial Urine (AU)===
Artificial urine prepared according to DIN EN 1616:1999, from Pickering Laboratories (Mountain View, USA).
Measure at 37° C, across concentration ranges:
AU-LiCl, AU-Li<sub>2</sub>CO<sub>3</sub>, AU-LiOr
===5. Artificial Cerebrospinal Fluid (aCSF)===