Changes

508 bytes added ,  19:12, 19 May 2009
no edit summary
Line 43: Line 43:  
  Reverse primer 5'-GTGAGGTTCTGGTTGTAGCTGACA-3'
 
  Reverse primer 5'-GTGAGGTTCTGGTTGTAGCTGACA-3'
 
  TaqMan probe 5'-FAM-TCACGGTGGTTGCCATCACTGTCTTC-TAMRA-3'
 
  TaqMan probe 5'-FAM-TCACGGTGGTTGCCATCACTGTCTTC-TAMRA-3'
 +
 +
 +
 +
 +
Primers chosen for experiment (according to Andre's advice)
 +
 +
(designed using [http://www.bioinformatics.nl/cgi-bin/primer3plus/primer3plus.cgi PrimerPlus3]
 +
 +
 +
 +
NR1  U11418.1
 +
2360 Forward ttcacagaagtgcgatctgg, Tm 60.0
 +
2467 Reverse atggacagggaaacgttctg, Tm 60.0
 +
Product size of 108 bp
 +
 +
 +
Serine Racemase    NM_198757
 +
 +
856 forward: tggtagatgcgttggtggta, Tm 60.0
 +
958  reverse: tcagcagcgtacaccttcac, Tm60.1
 +
 +
674-807, product size 134bp
 +
 +
Rat GAPDH
 +
 +
Forward TCACTGCCACTCAGAAGACTG
 +
Reverse GGATGACCTTGCCCACAGC
 +
 +
    
[[Category:Taste_Aversion]]
 
[[Category:Taste_Aversion]]