Difference between revisions of "DMH Sodium Butyrate and Saccharin CTA Controls"
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| + | Replication (to increase N) of [[DMF Sodium Butyrate and Saccharin-LiCl CTA]] and [[DMG Sodium Butyrate and Saccharin-LiCl CTA]] with NaCl-conditioned control groups. | ||
| + | {{Infobox experiment|align=right | ||
| + | | Name = Butyrate and Lithium-CTA pt3 | ||
| + | | project = Histone Acetylation | ||
| + | | code = DMH | ||
| + | | protocol = 1917 | ||
| + | | funding = Chair's Fund | ||
| + | | investigators = Mukherjee, Houpt | ||
| + | | species = rat | ||
| + | | date = 2021-1-19 | ||
| + | | type = CTA | ||
| + | | drugs = butyrate, lithium | ||
| + | | data = https://houptlab.org/bartab/expt/?id=DMH | ||
| + | }} | ||
| + | |||
| + | |||
| + | |||
| + | Test if systemic Sodium Butyrate (NaB), an HDAC inhibitor, enhances CTA learning . | ||
| + | |||
| + | A more strict replication of [https://houptlab.org/papers/pdfs/Houpt065.pdf Kwon and Houpt, Brain Research, 1333 (2010) 36–47], PMID: 20346924 [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2871962/ PMCID: PMC2871962]. | ||
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| + | 1. Sodium Butyrate ([https://www.sigmaaldrich.com/US/en/product/aldrich/303410 Sigma-Aldrich 303410-100G] Butyric acid sodium salt, 98% ). | ||
| + | |||
| + | 2. n=8 male, n=8 female, counterbalanced | ||
| + | |||
| + | |||
| + | Dose of NaB based on [https://houptlab.org/papers/pdfs/Houpt065.pdf Kwon and Houpt, Brain Research, 1333 (2010) 36–47], PMID: 20346924 [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2871962/ PMCID: PMC2871962] | ||
| + | |||
| + | * 0.3 M Na Butyrate = 3.3g/100ml water | ||
| + | * 0.3 M NaCl = 18g/liter water (osmotic control) | ||
| + | |||
| + | <br> | ||
| + | |||
| + | ==Data== | ||
| + | |||
| + | '''[https://houptlab.org/bartab/expt/?id=DMH Raw Data Link]''' | ||
| + | |||
| + | ==People== | ||
| + | |||
| + | Durba Mukherjee | ||
| + | |||
| + | |||
| + | ==CTA Conditioning== | ||
| + | |||
| + | ===Agenda=== | ||
| + | |||
| + | <br> | ||
| + | |||
| + | '''Rats arrive''' (January 12, 2021) | ||
| + | n=16 adult male (8) and female(8) sprague-dawley rats (Charles River CD), individually housed in BRF 169. | ||
| + | |||
| + | <br> | ||
| + | |||
| + | '''Day 0: Water removed''' (January 19, 2021) | ||
| + | |||
| + | <br> | ||
| + | |||
| + | '''Days 1 - 7: Water Restriction''' (January 20 - January 26, 2021) | ||
| + | |||
| + | Weigh rats daily. water for 10 min, ad lib food. | ||
| + | |||
| + | 10 min water at 1430 daily. | ||
| + | |||
| + | <br> | ||
| + | |||
| + | '''Day 8: Conditioning Day''' (January 27 2021) | ||
| + | |||
| + | 0 min: 30 min access to 0.125% saccharin (5 g sodium saccharin/4L water) | ||
| + | 30 min: all rats injected with 0.15 M NaCl, 3 ml/kg ip. | ||
| + | 40 min: half of rats (1-4, 9-12) injected with 0.3 M NaCl 12 ml/kg ip; other half of rats (5-8, 13-16) injected with 0.3 M Na Butyrate 12 ml/kg, ip | ||
| + | |||
| + | ad lib water returned after final injection overnight. | ||
| + | |||
| + | * Rats 1-8 : female, Rats 9-16: male | ||
| + | * All rats: saccharin | ||
| + | * All rats: NaCl (0.15M, 3ml/kg) | ||
| + | * rats 1-4, 9-12: NaCL injection; rats 5-8,13-16: NaB injection; | ||
| + | |||
| + | |||
| + | '''Days 9 - ??: Preference Tests''' (January 28 - ?? February , 2021) | ||
| + | |||
| + | Rats CTA acquisition and extinction was measured with 48-h, 2-bottle preference tests. Rats were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for ??? days. | ||
| + | |||
| + | |||
| + | [[Category:Taste Aversion]] | ||
| + | [[Category:CTA Experiments]] | ||
| + | [[Category:Epigenetics]] | ||
Latest revision as of 10:06, 5 July 2022
Replication (to increase N) of DMF Sodium Butyrate and Saccharin-LiCl CTA and DMG Sodium Butyrate and Saccharin-LiCl CTA with NaCl-conditioned control groups.
| Butyrate and Lithium-CTA pt3 | |
|---|---|
| Histone Acetylation | |
| Expt Code | DMH |
| Start Date | 2021-1-19 |
| Investigators | Mukherjee, Houpt |
| Funding | Chair's Fund |
| Protocol | 1917 |
| Expt Type | CTA |
| Species | rat |
| Drugs | butyrate, lithium |
| Data Link | https://houptlab.org/bartab/expt/?id=DMH |
Test if systemic Sodium Butyrate (NaB), an HDAC inhibitor, enhances CTA learning .
A more strict replication of Kwon and Houpt, Brain Research, 1333 (2010) 36–47, PMID: 20346924 PMCID: PMC2871962.
1. Sodium Butyrate ( Sigma-Aldrich 303410-100G Butyric acid sodium salt, 98% ).
2. n=8 male, n=8 female, counterbalanced
Dose of NaB based on Kwon and Houpt, Brain Research, 1333 (2010) 36–47, PMID: 20346924 PMCID: PMC2871962
- 0.3 M Na Butyrate = 3.3g/100ml water
- 0.3 M NaCl = 18g/liter water (osmotic control)
Data
People
Durba Mukherjee
CTA Conditioning
Agenda
Rats arrive (January 12, 2021) n=16 adult male (8) and female(8) sprague-dawley rats (Charles River CD), individually housed in BRF 169.
Day 0: Water removed (January 19, 2021)
Days 1 - 7: Water Restriction (January 20 - January 26, 2021)
Weigh rats daily. water for 10 min, ad lib food.
10 min water at 1430 daily.
Day 8: Conditioning Day (January 27 2021)
0 min: 30 min access to 0.125% saccharin (5 g sodium saccharin/4L water) 30 min: all rats injected with 0.15 M NaCl, 3 ml/kg ip. 40 min: half of rats (1-4, 9-12) injected with 0.3 M NaCl 12 ml/kg ip; other half of rats (5-8, 13-16) injected with 0.3 M Na Butyrate 12 ml/kg, ip
ad lib water returned after final injection overnight.
- Rats 1-8 : female, Rats 9-16: male
- All rats: saccharin
- All rats: NaCl (0.15M, 3ml/kg)
- rats 1-4, 9-12: NaCL injection; rats 5-8,13-16: NaB injection;
Days 9 - ??: Preference Tests (January 28 - ?? February , 2021)
Rats CTA acquisition and extinction was measured with 48-h, 2-bottle preference tests. Rats were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for ??? days.