DMF Sodium Butyrate and Saccharin-LiCl CTA

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Test if systemic Sodium Butyrate (NaB), an HDAC inhibitor, enhances CTA learning .

(see replication to increase N at DMG Sodium Butyrate and Saccharin-LiCl CTA)

A more strict replication of Kwon and Houpt, Brain Research, 1333 (2010) 36–47, PMID: 20346924 PMCID: PMC2871962.

1. Ordered brand new Sodium Butyrate ( Sigma-Aldrich 303410-100G Butyric acid sodium salt, 98% ).

2. n=8 male, n=8 female, counterbalanced


Dose of NaB based on Kwon and Houpt, Brain Research, 1333 (2010) 36–47, PMID: 20346924 PMCID: PMC2871962

  • 0.3 M Na Butyrate = 3.3g/100ml water
  • 0.3 M NaCl = 18g/liter water (osmotic control)


Data

Raw Data Link

People

Durba Mukherjee


CTA Conditioning

Agenda


Rats arrive (October 6, 2020) n=16 adult male (8) and female(8) sprague-dawley rats (Charles River CD), individually housed in BRF 169.


Day 0: Water removed (October 9, 2020)


Days 1 - 11: Water Restriction (October 10 - October 20, 2020)

Weigh rats daily. water for 10 min, ad lib food.

10 min water at 1430 daily.


Day 12: Conditioning Day (October 21, 2020)

0 min: 30 min access to 0.125% saccharin (5 g sodium saccharin/4L water) 30 min: all rats injected with 0.15 M LiCl, 3 ml/kg ip. 40 min: odd rats injected with 0.3 M NaCl 12 ml/kg ip; even rats injected with 0.3 M Na Butyrate 12 ml/kg, ip

ad lib water returned after final injection overnight.

  • Rats 1-8 : male, Rats 9-16: female
  • All rats: saccharin
  • All rats: LiCl (0.15M, 3ml/kg)
  • Odd rats: NaCL injection; Even rats: NaB injection;


Days 13 - 22: Preference Tests (October 22 - November 1, 2020)

Rats CTA acquisition and extinction was measured with 48-h, 2-bottle preference tests. Rats were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 10 days.