Difference between revisions of "DMF Sodium Butyrate and Saccharin-LiCl CTA"
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| − | Test if systemic Sodium Butyrate (NaB), an HDAC inhibitor, enhances CTA learning . | + | Test if systemic Sodium Butyrate (NaB), an HDAC inhibitor, enhances CTA learning. |
| + | |||
| + | {{Infobox experiment|align=right | ||
| + | | Name = Butyrate and Lithium-CTA pt1 | ||
| + | | project = Histone Acetylation | ||
| + | | code = DMF | ||
| + | | protocol = 1917 | ||
| + | | funding = Chair's Fund | ||
| + | | investigators = Mukherjee, Houpt | ||
| + | | species = rat | ||
| + | | date = 2020-10-9 | ||
| + | | type = CTA | ||
| + | | drugs = butyrate, lithium | ||
| + | | data = https://houptlab.org/bartab/expt/?id=DMG | ||
| + | }} | ||
| + | |||
(see replication to increase N at [[DMG Sodium Butyrate and Saccharin-LiCl CTA]]) | (see replication to increase N at [[DMG Sodium Butyrate and Saccharin-LiCl CTA]]) | ||
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'''Days 13 - 22: Preference Tests''' (October 22 - November 1, 2020) | '''Days 13 - 22: Preference Tests''' (October 22 - November 1, 2020) | ||
| − | Rats CTA acquisition and extinction was measured with | + | Rats CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests. Rats were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 10 days. |
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[[Category:Taste Aversion]] | [[Category:Taste Aversion]] | ||
| + | [[Category:CTA Experiments]] | ||
| + | [[Category:Epigenetics]] | ||
Latest revision as of 11:03, 5 July 2022
Test if systemic Sodium Butyrate (NaB), an HDAC inhibitor, enhances CTA learning.
| Butyrate and Lithium-CTA pt1 | |
|---|---|
| Histone Acetylation | |
| Expt Code | DMF |
| Start Date | 2020-10-9 |
| Investigators | Mukherjee, Houpt |
| Funding | Chair's Fund |
| Protocol | 1917 |
| Expt Type | CTA |
| Species | rat |
| Drugs | butyrate, lithium |
| Data Link | https://houptlab.org/bartab/expt/?id=DMG |
(see replication to increase N at DMG Sodium Butyrate and Saccharin-LiCl CTA)
A more strict replication of Kwon and Houpt, Brain Research, 1333 (2010) 36–47, PMID: 20346924 PMCID: PMC2871962.
1. Ordered brand new Sodium Butyrate ( Sigma-Aldrich 303410-100G Butyric acid sodium salt, 98% ).
2. n=8 male, n=8 female, counterbalanced
Dose of NaB based on Kwon and Houpt, Brain Research, 1333 (2010) 36–47, PMID: 20346924 PMCID: PMC2871962
- 0.3 M Na Butyrate = 3.3g/100ml water
- 0.3 M NaCl = 18g/liter water (osmotic control)
Data
People
Durba Mukherjee
CTA Conditioning
Agenda
Rats arrive (October 6, 2020) n=16 adult male (8) and female(8) sprague-dawley rats (Charles River CD), individually housed in BRF 169.
Day 0: Water removed (October 9, 2020)
Days 1 - 11: Water Restriction (October 10 - October 20, 2020)
Weigh rats daily. water for 10 min, ad lib food.
10 min water at 1430 daily.
Day 12: Conditioning Day (October 21, 2020)
0 min: 30 min access to 0.125% saccharin (5 g sodium saccharin/4L water) 30 min: all rats injected with 0.15 M LiCl, 3 ml/kg ip. 40 min: odd rats injected with 0.3 M NaCl 12 ml/kg ip; even rats injected with 0.3 M Na Butyrate 12 ml/kg, ip
ad lib water returned after final injection overnight.
- Rats 1-8 : male, Rats 9-16: female
- All rats: saccharin
- All rats: LiCl (0.15M, 3ml/kg)
- Odd rats: NaCL injection; Even rats: NaB injection;
Days 13 - 22: Preference Tests (October 22 - November 1, 2020)
Rats CTA acquisition and extinction was measured with 24-h, 2-bottle preference tests. Rats were given 24-h free access to both 0.125% saccharin and water bottles, alternating position for 10 days.