Difference between revisions of "Serotonin Genes"
(listed TPH2 primers) |
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I am interested in 4 of the genes related to serotonin function in depression and food intake: | I am interested in 4 of the genes related to serotonin function in depression and food intake: | ||
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=PCR primers= | =PCR primers= | ||
| − | Tryptophan Hydroxylase 2 cDNA for in situ hybridization | + | ==Tryptophan Hydroxylase 2 cDNA== |
| + | |||
| + | for in situ hybridization | ||
product size: bp, range & ascension number? | product size: bp, range & ascension number? | ||
| − | TPH2for | + | :TPH2for: 5’ -GAAGTTGGCGGGCTGGTGAGA–3’ |
| − | TPH2rev | + | |
| + | :TPH2rev: 5’ -TCGGCAAGCATGAGTGGGGTAGA–3’ | ||
Reference: Kulikov AV, Osipova DV, Naumenko VS, Popova NK. Association between Tph2 gene polymorphism, brain tryptophan hydroxylase activity and aggressiveness in mouse strains. Genes Brain Behav. 2005 Nov;4(8):482-5. PMID 16268992 | Reference: Kulikov AV, Osipova DV, Naumenko VS, Popova NK. Association between Tph2 gene polymorphism, brain tryptophan hydroxylase activity and aggressiveness in mouse strains. Genes Brain Behav. 2005 Nov;4(8):482-5. PMID 16268992 | ||
| − | Tryptophan Hydroxylase 2 | + | ==Tryptophan Hydroxylase 2 polymorphisms== |
| + | |||
| + | linked to aggression. 5 min at 94°C then 40 cycles (30 s at 94°C, 30 s at 60°C, 30 s at 72°C) | ||
Positive control, product size 523 bp | Positive control, product size 523 bp | ||
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| − | |||
| − | 1473C allele | + | :ctrlTPH2for: 5’ -tttgacccaaagacgacctgcttgca –3’ |
| + | |||
| + | :ctrlTPH2rev: 5’-tgcatgcttactagccaaccatgagaca-3’ | ||
| + | |||
| + | :1473C allele, product size 307bp | ||
| + | |||
TPH2C1473: 5’-cagaatttcaatgctctgcgtgtggg-3’ | TPH2C1473: 5’-cagaatttcaatgctctgcgtgtggg-3’ | ||
| − | 1473G allele | + | :1473G allele. product size 307 bp |
| + | |||
TPH2g1473: 5’-cagaatttcaatgctctgcgtgtggc-3’ | TPH2g1473: 5’-cagaatttcaatgctctgcgtgtggc-3’ | ||
Reference: Zhang X, Beaulieu JM, Sotnikova TD, Gainetdinov RR, Caron MG. Tryptophan hydroxylase-2 controls brain serotonin synthesis. Science. 2004 Jul 9;305(5681):217. PMID 15247473 | Reference: Zhang X, Beaulieu JM, Sotnikova TD, Gainetdinov RR, Caron MG. Tryptophan hydroxylase-2 controls brain serotonin synthesis. Science. 2004 Jul 9;305(5681):217. PMID 15247473 | ||
Revision as of 12:57, 24 September 2007
I am interested in 4 of the genes related to serotonin function in depression and food intake:
Pet-1
Serotonin Transporter
Tryptophan Hydroxylase 2
5HT2C Receptor
PCR primers
Tryptophan Hydroxylase 2 cDNA
for in situ hybridization
product size: bp, range & ascension number?
- TPH2for: 5’ -GAAGTTGGCGGGCTGGTGAGA–3’
- TPH2rev: 5’ -TCGGCAAGCATGAGTGGGGTAGA–3’
Reference: Kulikov AV, Osipova DV, Naumenko VS, Popova NK. Association between Tph2 gene polymorphism, brain tryptophan hydroxylase activity and aggressiveness in mouse strains. Genes Brain Behav. 2005 Nov;4(8):482-5. PMID 16268992
Tryptophan Hydroxylase 2 polymorphisms
linked to aggression. 5 min at 94°C then 40 cycles (30 s at 94°C, 30 s at 60°C, 30 s at 72°C)
Positive control, product size 523 bp
- ctrlTPH2for: 5’ -tttgacccaaagacgacctgcttgca –3’
- ctrlTPH2rev: 5’-tgcatgcttactagccaaccatgagaca-3’
- 1473C allele, product size 307bp
TPH2C1473: 5’-cagaatttcaatgctctgcgtgtggg-3’
- 1473G allele. product size 307 bp
TPH2g1473: 5’-cagaatttcaatgctctgcgtgtggc-3’
Reference: Zhang X, Beaulieu JM, Sotnikova TD, Gainetdinov RR, Caron MG. Tryptophan hydroxylase-2 controls brain serotonin synthesis. Science. 2004 Jul 9;305(5681):217. PMID 15247473