Changes

I am interested in 4 of the genes related to serotonin function in depression and food intake:

I am interested in 4 of the genes related to serotonin function in depression and food intake:

=PCR primers=

=PCR primers=

Tryptophan Hydroxylase 2 cDNA for in situ hybridization

==Tryptophan Hydroxylase 2 cDNA==

 

for in situ hybridization

product size:  bp, range & ascension number?

product size:  bp, range & ascension number?

TPH2for GAAGTTGGCGGGCTGGTGAGA 21 bases

:TPH2for: 5’ -GAAGTTGGCGGGCTGGTGAGA–3’

TPH2rev TCGGCAAGCATGAGTGGGGTAGA 23 bases

 

:TPH2rev: 5’ -TCGGCAAGCATGAGTGGGGTAGA–3’

Reference: Kulikov AV, Osipova DV, Naumenko VS, Popova NK. Association between Tph2 gene polymorphism, brain tryptophan hydroxylase activity and aggressiveness in mouse strains. Genes Brain Behav. 2005 Nov;4(8):482-5. PMID 16268992

Reference: Kulikov AV, Osipova DV, Naumenko VS, Popova NK. Association between Tph2 gene polymorphism, brain tryptophan hydroxylase activity and aggressiveness in mouse strains. Genes Brain Behav. 2005 Nov;4(8):482-5. PMID 16268992

Tryptophan Hydroxylase 2 polymorphism linked to aggression

==Tryptophan Hydroxylase 2 polymorphisms==

 

linked to aggression.  5 min at 94°C then 40 cycles (30 s at 94°C, 30 s at 60°C, 30 s at 72°C)

Positive control, product size 523 bp

Positive control, product size 523 bp

1473C allele: 307bp

 

 

:1473C allele, product size 307bp

 

TPH2C1473: 5’-cagaatttcaatgctctgcgtgtggg-3’

TPH2C1473: 5’-cagaatttcaatgctctgcgtgtggg-3’

1473G allele : 307 bp

:1473G allele. product size 307 bp

 

TPH2g1473: 5’-cagaatttcaatgctctgcgtgtggc-3’

TPH2g1473: 5’-cagaatttcaatgctctgcgtgtggc-3’

Reference:  Zhang X, Beaulieu JM, Sotnikova TD, Gainetdinov RR, Caron MG. Tryptophan hydroxylase-2 controls brain serotonin synthesis. Science. 2004 Jul 9;305(5681):217. PMID 15247473

Reference:  Zhang X, Beaulieu JM, Sotnikova TD, Gainetdinov RR, Caron MG. Tryptophan hydroxylase-2 controls brain serotonin synthesis. Science. 2004 Jul 9;305(5681):217. PMID 15247473