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C-Fos Methylation (view source)

334 bytes added , 16:16, 23 January 2009

→‎Rat c-fos Primers: added our current primers

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==Rat c-fos Primers==

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==Rat c-fos Primers used by Alcala and Houpt==

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Forward:

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Reverse:

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Product size:

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PCR conditions for use with HotStarTaq mix (progam CFOSGENO on thermocycler)

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95° for 15 min to activate Taq

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Repeat 35 times:

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:94° for 1 min

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:59° for 1 min

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:72° for 1 min

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:72° for 10 min final extension

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4° for overnight

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==Rat c-fos Primers from Literature==

From Shimizu et al., Cancer Sci 2007; 98: 1318–1322

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