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→Rat c-fos Primers used by Alcala and Houpt: added actual sequences
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==Rat c-fos Primers used by Alcala and Houpt==
==Rat c-fos Primers used by Alcala and Houpt==
+Primers designed with Primer 3 using the 300 bases upstream of c-Fos transcription start based on gi|62750806|ref|NC_005105.2|NC_005105
+(equivalent to bases 172-471 of U02631, which claims start of 5’UTR is at 455.)
−Forward:
+Forward at -261 (cFosSense-261):
+:5'-CCATCCCCGAAATCCTACAT-3'
−Reverse:
+Reverse at -6 (cFosAS-6):
+:5'-CAATCGCGGTTGGAGTAGTA-3'
−Product size:
+Product size: 256 bp
PCR conditions for use with HotStarTaq mix (progam CFOSGENO on thermocycler)
PCR conditions for use with HotStarTaq mix (progam CFOSGENO on thermocycler)
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4° for overnight
4° for overnight
−==Rat c-fos Primers from Literature==
==Rat c-fos Primers from Literature==