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321 bytes added ,  17:23, 23 January 2009
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==Rat c-fos Primers used by Alcala and Houpt==
 
==Rat c-fos Primers used by Alcala and Houpt==
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Primers designed with Primer 3 using the 300 bases upstream of c-Fos transcription start based on gi|62750806|ref|NC_005105.2|NC_005105
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(equivalent to bases 172-471 of U02631, which claims start of 5’UTR is at 455.)
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Forward:
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Forward at -261 (cFosSense-261):
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:5'-CCATCCCCGAAATCCTACAT-3'
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Reverse:
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Reverse at -6 (cFosAS-6):
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:5'-CAATCGCGGTTGGAGTAGTA-3'
   −
Product size:
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Product size: 256 bp
    
PCR conditions for use with HotStarTaq mix (progam CFOSGENO on thermocycler)
 
PCR conditions for use with HotStarTaq mix (progam CFOSGENO on thermocycler)
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4° for overnight
 
4° for overnight
      
==Rat c-fos Primers from Literature==
 
==Rat c-fos Primers from Literature==

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